50 



in alkaline beef broth. Ps. steivartl grew in this broth without 

 retardation. Three .saprophytic bacteria, obtained by Mr. A. F. 

 Woods from the surface of carnation leaves, also clouded this broth 

 in 2 to 7 days, viz, a pink buff germ, a lemon yellow germ, and an 

 orange colored germ, the latter probably identical with Bacterium 

 diaiithi AYt\\\x\' and Bolley. (See also Growth in fluid media.) 



Cabbagk Juice. 



This fluid was prepared by grinding green cabbage leaves and 

 extracting the juice under pressure. No water was added. The leaves 

 were from old, slow-growing, hothouse plants. This juice was 

 divided into two portions, one of which was sterilized by forcing it 

 through a Chamberland filter, and the other b}^ steaming for a few 

 minutes on 3 consecutive da} s. There was no difference in the acidity, 

 each titrating +40 with caustic soda and phenolphthalein. The boiled 

 juice smelled strongly of cabbage. Each stock was inoculated in the 

 same way, i. e., with a small mass of bright yellow slime from a 

 starch-jelh' culture 28 days old. The tubes were well plugged and 

 set in a dark place exposed to room temperatures of 22° to 33° C. 

 (mostly 25° to 29°). 



Result. — One tube of the filtered juice was under observation 44 

 days, but no growth appeared. Two tubes of the boiled juice were 

 under observation, respectivel}", 29 and 44 daj^s, but there was no 

 growth. Five tubes of slant agar were inoculated at the same time 

 from the same culture, and all took readih'. Knowing that bac- 

 teria will tolerate more acid in a solid than in a fluid medium, 150 

 mgs. of Lautenschlager's neutral agar flour was added to one of 

 the tubes on the twenty-ninth day. This was then steam sterilized, 

 slanted, and the surface carefull}" streaked with at least a cubic milli- 

 meter of bright yellow slime from an agar culture 4 da^^s old. This 

 slant culture was under observation, in conditions favorable to growth, 

 for 45 days, but no growth ensued, except on the wall of the tube above 

 the slant in a place which Avas accidentalh" touched by the loop and 

 where a little moisture condensed. 



Ps. 2)J(CLseoh' and Ps. campestris also refused to grow in this acid 

 cabbage juice; but when the fluid was solidified b}" adding 150 mgs. of 

 the agar flour the latter made a ver}- copious and prolonged growth — 

 i. e., much better than on ordinar}- agar, although it was started upon 

 it with great difliculty (3 copious inoculations). On the contrary. 

 Bacillus amylovorus and Ps. stewarti grew in the boiled juice without 

 retardation. The latter, inoculated from a solid culture, clouded the 

 fluid (2 tubes) in less than 48 hours iind made a ver}' prolonged and 

 copious growth. B. amylovorus grew nearl}' as well. 



