105 



confused with any uudisMolved slime uHcd in making tlic inoculafinn. At the end of 

 26 hours there was a slight growth on the e>'linder in eaeh tube. On the third day, 

 in the thermostat (temperatures 34.45° p. m., 34..35° a. m., 38.35° p. m., 34.53° a. m., 

 34.15° p. m., 34.32° a. m.) the germs covered 2 s(j. cm. on one side of the cylinder. 

 This growth was jjlainly yellow but extremely thin. On tlie fifth day (temperatures 

 34.32° p. m., 35° a. m., 34.40° p. m., 34.85° a. m.) there seemed to be a slight 

 increase in growth. This growth was very thin, distinctly yellow, not smooth, and 

 rather dry, i. e., not wet-shining. In the check tube there was from 10 to 20 times 

 as much growth, but not as much growth as there should have been, owing to the 

 fact that the check cylinder was rather dry. On the eighth daj^ (temperatiires 34.85° 

 a.m., 34.55° p.m., 35.55° a.m., 35.45° p.m., 34.83° a.m., 34.65° p.m., 34.95° a.m.) 

 there was some increase, the growth being distinctly yellow, but too thin to hide 

 minute irregularities of the substratum. The ^'olume of growth at this time was not 

 one-fiftieth that in the check tuVje. Examined microscopically, this growth con- 

 sisted of zoogloete, short rods and long rods. The short rods were single, in doubles, 

 or in fours; the long rods were slender threads, 10 to 20 or more times the length of 

 an ordinary rod. These threads were numerous and their segments were not well 

 defined. No involution forms were observed or anybodies suggestive of spores. On 

 the twelfth day (temperatures .34.05° a. m., 33.35° p. m., 34.45° a. m., 33.35° p. m., 



32.75° a. m., 32.45° p. m., a. m. ) the growth was meager, thin, dull yellow, and 



its surface was shagreened. There was no yellow slime in the water, but the germs 

 on the cylinder out of the water appeared as if still growing, although very slowly. 

 After 49 days in the thermostat a large loop of slime from this tujje was removed and 

 put into alkaline beef broth. This tube was kept at room temperatures for 27 days, 

 but n(j gro\\-th ensued. 



(11) This experiment was undertaken to see ij cultures started at room tempera- 

 tures would not do better when put into the thermostat than those which had been 

 inserted soon after inoculation. For this purpose I selected a tube of alkaline beef 

 broth, which had been kept as a check on series No. S, and a tube of yellow turnip, 

 kept as a check on series No. 9. The turnip culture was put into the thermostat on 

 the fifth day, at which time there was a coinous, yellow, wet-shining, homogeneous- 

 looking growth covering most of that part of the cylinder out of the water. The tube 

 of beef broth was put in on the eighth day, at which time the fluid was moderately 

 cloudy and had thrown down a little yellow precipitate, but had not yet developed 

 any pellicle, rim of germs, or zooglceae. The temperatures were 34.15° to 35.55° dur- 

 ing the first 9 days (once as low as 33.35°) and then 32.45° to 34.45° C. There was 

 no exact check tube for the turnip, but a transfer was made from it into another tube 

 of the same medium; for comparison witli the beef l)roth the other check tube of 

 series No. 8 was used. 



Result: (a) The beef broth in the thermostat at once fell behind the check tube 

 in growth. On the fifth day the clouding appeared to be feebler than on the start 

 and the trifling precipitate had increased proportionately to the decrease in clouding, 

 but scarcely more. The check tulje was distinctly cloudier. On the ninth day there 

 was no increase of precipitate. On the twenty-ninth day there was no pellicle, no rim 

 of germs, no zoogloeae, and not more precipitate than on the fifth day, i. e., there 

 appeared to have been no growth whatever during the whole time of the exposure. 

 On this date the check tube was uniformly clouded, showed a yellow rim, and had 

 thrown down a yellow precijjitate 12 mm. Ijroad and 2 mm. deep. On the twenty- 

 ninth day a large loop of fluid was taken from the tube in the thermostat and put 

 into a sterile tube of the same l)eef broth. This tube was under observation 17 days, 

 in conditions very well suited for growth, ])ut no growth ensued. At the end of 46 

 days in the thermostat this experiment was repeated, inoculating copiously into 

 alkaline beef broth diluted with distilled water. The tube was kept at room tem- 

 peratures and watched for 27 days, but no growth ensued, i. e., no spores were pres- 



