]06 



ent. (h) On thf fiftli day in the thermostat the slime on the turnip cylinder was 

 still \vet-.«hinincr, but it ^va^; not as homogeneous looking, being uniformly mottled 

 lighter and darker yellow. On the eighth day the culture was less vigorous and the 

 substratum had browned slightly. The slime was now examined microscopically 

 for several liours. It consisted of the ordinary short rods and of slender threads 

 which were of the same diameter as the rods but were often 50 times as long. These 

 threads were numerous. No involution forms were observed injr any bodies resem- 

 bling spores. On the tw'elfth day the culture was in a much worse condition. Growth 

 had ceased and the slime out of the water had so much dried out that the substratum 

 under it was now visiljle. Nineteen days after this date the turnij) cylinder which 

 had been inoculated froju this tube and kept at room temijeratures was still covered 

 with a thick, smooth, wet-shining, homogeneous-looking, pale yellow layer of slime, 

 entirely hiding the substratum. The fluid in the bottom of the tube was also grown 

 full of the slime, which was not the case with the culture in the thermostat. After 

 49 days in the thermostat a large loop from this tube was put into alkaline beef 

 broth and watched at room temperatures for 27 days, but no growth ensued. 



(12) Two steamed cylinders of carrot (stock 290), standing in several cubic centi- 

 meters of distilled water in tubes of resistant glass, were each inoculated with a large 

 loop of the yellow slime of Ps. hi/dcinlhi from recent growths in a turnip culture 5 

 days old. These tubes were then shaken until the slime was dissolved in the water 

 and washed over the cylinder. One of the tubes was put into the thermostat at 

 33.35° to 35.45° C. (mostly 34.35° to 35°) and the other was held at room temperatures. 



Result: On the thir<l day the check tulte showed a plentiful yellow growth, cover- 

 ing nearly all of one side of the long cylinder. On the fifth day this growth was 

 dense enough to hide the orange color of the substratum. The tube in the thermostat, 

 on the eighth day, showed no growth whatever, although it still held 2 c. c. of water 

 and was consequently moist. This tuloe was now removed to room temperatures of 

 19° to 25° C. On the fourth day thereafter a copious, smooth, wet-shining, homo- 

 geneous-looking, bright yellow growth, dense enough tri liide the substratum, covered 

 about 3 sq. cm. of the inoculated cylinder. 



(13) Two cylinders from a yellow, flat-bottomed turnip, prepared in the same way 

 as the carrot, were inoculated at the same time as the latter and from the same culture, 

 each tube receiving a large loop of the yellow slime. These tubes were then shaken 

 until the slime was dissolved in the water and spread over the cylinder. One of the 

 tubes was held at room temperatures and the other was put into the thermostat. 

 The tube in the thermostat contained several cubic centimeters of water; the check 

 tul)e contained only a small amount of water. 



Result: On the third day, in the dieck tube, there was a copious, smooth, wet- 

 shining, yellow growth over nearly the entire cylinder. On the fifth day this growth 

 had become more abundant, covering the whole cylinder and flllihg up the small 

 amount of fluid in the bottom of the tube. The other tube was left in the thermostat 

 8 days at 33..35° to 35.55° (mostly 34.35° to 35°), during which time no growth was 

 visible either to the naked eye or with a Zeiss X 6 aplanat. The tube was now 

 removed to room temperatures of 20° to 25° C. On the fourth day after this removal 

 two-thirds of the cylinder (all out of the water) was covered with a copious, yellow, 

 smooth, wet-shining, homogeneous-looking bacterial layer, which developed nor- 

 mally for Ps. hyacinthi. 



(14) Four tubes of 1: 2 acid beef broth (stock 286a, acidity + 25), originally hold- 

 ing exactly 10 c. c, but dried out about one-fifth by long standing and consequently 

 more acid than the original stock, were each inoculated with two large loops from 

 an alkaline beef broth culture of Ps. lujacinthi 10 days old. This culture was uni- 

 formly clouded, and showed considerable yellow precipitate, but there were no 

 zoogloea? and the rim of germs was only commencing to form, i. e., the fluid was 

 crowded full of living germs and in excellent condition for use. Two of the tubes 



