150 



Growth on steamed 

 potato cylinders 

 standing in dis- 

 tilled water. 



Growth in milk 



Growth in litmus 

 milk. 



Growth on nutrient fl. 

 gelatin and Loeff-| 

 ler's l:)lood serum. i2. 

 fl. 



Growth. on steamed 

 yellow turnips or 

 rutabagas standing 

 in distilled water. 



1. 

 Growth in milk or 



bouillon with 



ethyl alcohol. 



Behavior in tomato rl. 

 juice and cabbagej 2. 

 juice. ' 



Behavior in concen-il. 

 trateil beel brothj 2. 

 (acidity, -rSO) . I 

 1. 



Behavior in Dun- 

 ham's solution 

 with indigo car- 

 mine. 



Behavior in Dun- 

 ham's solution 

 with methylene 

 blue. 



Copious and prolonged, covering the potato and filling the 

 water with a solid yellow slime and changing all of the 

 starch within a few weeks so that it does not react blue 

 or red with alcohol iodine or iodine potassium iodide. Ps. 

 campestris, Ps. phaseoli. 



Moderate and very little after the second week, not always 

 covering all of the exposed part of the potato and never fill- 

 ing the water with a solid yellow slime, the starch but little 

 acted upon and always yielding (even immediately under 

 the slime) a pronounced blue, blue purple, or red purple 

 reaction. Ps. hyacintki, Ps. steirarti. 



The whey is slowly separated from the casein by means of a 

 lab ferment; the casein slowly settles and after some weeks 

 is partially redissolved. Ps. campestris, Ps. lihaseoli, Ps. 

 hyacinthi. 



Growth good, but milk continues opaque and the whey never 

 separates from the casein. Ps. stewarti. 



Blue litmus becomes gradually more and more alkaline. At 

 no time is there any indication of acids. Ps. canipestm, 

 Ps. phaseoli, Ps. hyacinthi. 



Blue litmus in course of some weeks changes to lilac or helio- 

 trope, indicating the formation of a slight amount of acid. 

 Ps. steirarti. 



A slow liquefaction, best in the order named. Ps. phaseoli, 

 Ps. rampestris, Ps. hyacinthi. The latter brightest yellow. 



A good buff-yellow growth, but no liquefaction. Ps. steirarti. 



Copious in the air and filling the fluid with a thick yellow 

 slime, which is not iridescent; substratum browned and 

 softened. Ps. campestris, Ps. hyacinthi. The latter Naples 

 yellow, the former paler yellow. 



Buff yellow, slightly iridescent, sparing (thin), and soon at 

 an end, never filling the water with a solid yellow slime. 

 Su})Stratum not browned or softened. Ps. steirarti. 



On 1 )oiling old cultures the steam yields an acid reaction and 

 a fragrant smell. Ps. hyacinthi. 



No such acid reaction or odor. Ps. campestris, Ps. phaseoli, 

 Ps. i^teirarti. 



Did not grow. P^. cainpestri.% Ps. phaseoli, Ps. hyacinthi. 



Grew copiously and for a long time without retardation 

 (cabbage) or with only a slight retardation. Ps. .stewarti. 



No growth. Ps. campeMris, Ps. phaseoli, P^. hyacinthi. 



Retardation for some days, then a copious and prolonged 

 growth. Ps. stewarti. 



No immediate reduction; color slowly changes to a pure 

 bright blue, which persists for several weeks, but finally 

 fades through green to yellowish. Ps. hyacinthi. 



No immediate reduction; color bluer for a few days only, 

 changing to green and bleaching much sooner than the 

 preceding. Ps. carnpeslri.-<, Ps. steirarti. 



Marked reduction; on shaking, a prompt reoxidation (to 

 blue) ; final color the .same as at the beginning (pure blue); 

 liacterial precipitate not stained. Ps. hyacinthi. 



As above, but the final color of the fluid green. Ps. campes- 

 tris. 



No reduction, final color of the fluid blue; bacterial precipi- 

 tate stained deep blue. Ps. steirarti. 



