The Bulletin 27 



tested on susceptible pigs. The test is made by injecting two cubic 

 centimeters of virus into each of four susceptible pigs (25 to 35 pounds) 

 preferably from the same litter. These pigs are then injected with 

 ■different amounts of serum. No. 1 would get two cubic centimeters of 

 virus and twenty cubic centimeters of serum ; 'No. 2, fifteen cubic centi- 

 meters of serum and two cubic centimeters of virus. No. 3, ten cubic 

 centimeters of serum and two cubic centimeters of virus ; No. 4 would get 

 two cubic centimeters of virus and no serum. If No. 4 dies within 

 fifteen days and Nos. 1, 2, and 3 show no signs of sickness, we then know 

 that the virus used was virulent and that the serum protected Nos. 1, 2, 

 and 3 from what would have been a fatal dose of virus. 



Directions for Ordering Serum. 



The serum will be shipped, by express, C.O.D., to any one ordering 

 it, unless check or money order accompanies the order. Do not fail to 

 give correct address. 



Always state correctly the amount of serum wanted, or give the 

 weight of each hog to be treated. If a hypodermic syringe is desired, 

 state so in your order, otherwise it will not be sent. A twenty cubic 

 centimeter glass barreled syringe will be sent at cost, $1.75, if ordered. 



The serum will be shipped in the following size bottles : 



30 c.c, 50 c.c, 100 c.c, 120 c.c, 150 c.c, ISO c.c, 200 c.c, 250 c.c, 

 500 c.c, 750 c.c, and 1000 c.c. 



The cost of the serum is one and one-quarter cents per cubic centimeter. 

 No serum will be taken back; when the serum is placed in the exj^ress 

 office it becomes your serum. 



Address all communications for serum to the State Veterinarian, 

 Department of Agriculture, Raleigh, N. C. 



Vaccination Doses. 



It requires more serum per pound of weight to "immunize" young 

 pigs than is required to "immunize" older hogs. 



Cholera-free Herds. Infected Herds. 



Suckling pigs 5 to 10 c.c. 10 to 15 c.c. 



25 to 50 pounds 20 c.c. 25 c.c. 



50 to 100 pounds 25 c.c 30 cc. 



100 to 150 pounds 30 cc 40 c.c. 



150 to 200 pounds 40 c.c. 50 c.c. 



■200 to 250 pounds 50 c.c. 60 c.c 



250 to 300 pounds 60 cc. 70 c.c. 



300 to 350 pounds 65 c.c. 75 cc 



350 to 400 pounds 70 cc 80 cc. 



All over 400 pounds 80 c.c. 90 c.c. 



Penalty for Allowing Diseased Hogs to Run at Large. 



"If any person having swine affected with the disease known as hog 

 cholera, or any other infectious or contagious disease, and discovering 

 the same, or to whom notice of the fact shall be given, shall fail of neg- 



