Distribution of natural hormones 



In chromatograms sprayed with Ehrlich reagent, an intense bkie spot due 

 to lAA always developed in strip 2. Immediately behind this there were 

 often two more faint and diffuse spots, a pinkish region of i?^ approx. 0-14 and 

 a greenish blue one of R^ 0-10. It was difficult to separate tliese completely 

 from the lAA but our results would suggest that they were either inactive or 

 only weakly active in the wheat cylinder test. 







SO 



i 



t 



i 



1 



i 



Experiment A 

 Feb. 1955 



Experiment B 

 May 1955 



2\\n 



2V\\rh 



72\\r% 



-Rf i/alue- 



lAA zone 



Inhibitor zone 



1-0 



120 hn 



168 hrs 



Figure 5. Biological assay of hormones and inhibitors in extracts from germinating peas, var. Alaska. 

 {Acid fractions.) 



Experiments with peas 



Similar experiments have been carried out with pea seeds — a typical 

 non-endospermic dicotyledon. In these experiments the variety 'Alaska' was 

 used and the methods were similar to those described for maize. The results 

 of two experiments with peas are presented in Figure 5. 

 From the results the following observations can be made: 

 (a) At all stages the lAA content is much lower than in maize. In the 

 early stages of germination it is extremely low and it increases slowly as 

 germination proceeds. 



37 



