Metabolism and mode of action 



Water 



/A A 



lAAm 



IAN 



lAMe 



fif values 

 0-0-1 0-1-0-2 0-2-0-3 0-3-0-'^ O-f-O-S 0-5-0-6 0-6-0-7 0-7-0-8 O-B-0-9 0-3-1-0 



Solutions treated wlffi celery tissue 

 Figure 15. Bio-assay of chromatogram of extracts from celery-treated solutions using the pea curvature 



test. 



^ 



0-1 

 0-2- 

 K)-3— I 



0-4 







•0'6 



0-7- 

 ■0-8- 

 ■0-9' 



'Bnown\ 

 \l 



Water 



Pale 

 brown 



:L 



Yellow 



lAA 



I \ 



I \ 



;Brown\ 



ilue 



Pale 



brown 



L 



Yellow 



I A Am 



—I — r- 

 / \ 

 / \ 



I Brown^ 



BTuF 



TT 



TRJf 



I Pale 



brown 



Blue 



IAN 



-V- 

 Brown \ 



[Pale 

 brown 



Yellow 



Grej' 



lAMe 



n — r 

 / \ 



/Brcwn\ 



Blue 



; 



I Pale 

 I brown' 



Yellow 



Blue 



Authentic 

 compounds 



Red 



Blue 



Blue 



— Grey 

 Blue — 



ICA 

 lAA 



lAAm 



IAN 

 lAMe 



Figure 16. Diagrammatic representation of a colour chromatogram of extracts from solutions treated 

 with maize tissue. 



The chromatogram was developed and treated as described in Figure 3, 



of the 0-8-0-9 portion of the paper was inhibited by toxicity which can be 

 attributed to a high concentration of IAN. 



The colour chromatogram of solutions treated with maize coleoptiles 

 {Figure 16), although masked by brown pigments extracted from the tissue, 



244 



