1762 



Bassham, Benson, Kay, Harris, Wilson and Calvin 



Vol. 76 



DEORAnATION OF SbDOHBPTULOSR 



H 



H 



HC=N— N— CJI. 



L 



t=N— N— C^H, 

 I H 



HOCH 



HIO, 



NaHCO, 



Phenyl- 

 hyrirszine 



Ha 



CHjOH 



I 

 HOCH 



I 

 HCOH 



\ 

 HCOH 



HCOH 



CHjOH 



H, 

 PtO, 



CH,OH 

 HCOH 

 HOCH 

 HCOH 



HCOH 



I 

 HCOH 



1 

 CHjOH 



Dowex-50 

 100° 



NalO. 



HC=N— N— C«Hi 



C==N— N— C,Hj + 3HC00H + HCHO 

 I H 



CHO 



1, 2, 3 4. 5, 6 7 



CH,OH 



I 



-C 1 



CHO I 

 b CHO () 



I i 



•— CH 



I 

 HjC 



HCOOH 

 4 



+ 



H-i 



+ 



Acetobacter 

 suboxydans 



H,C 



CH.OH 

 HOCH 

 HOCH 



HCOH 



HCOH 



HCOH 



I 

 CH2OH 



Acelokacter 



CeCClO,),- 



CO, 



2 



6HC00H 



HIO, 



suboxydans 



W 



Ce(CIO,),- 



CHiOH 



I 

 HCOH 



I 

 HOCH 



I 

 HCOH 



HCOH 



I 



c=o 



I 



CHaOH 

 Guloheptulose 



ml. /minute and observing the spreading of color during its 

 travel through the tubing. For the longest length of tubing 

 used, the dye was seen to reach the end of the tubing be- 

 tween 14 and 17 seconds, and at a shorter time between 9 

 and 11 seconds, so that the spread of flow in time appeared 

 to be about 20% of the flow time. The times given are 

 average times of exposure of the algae to C*. Use of the 

 dye also permitted observation of the mixing of C'Oj solu- 

 tkm with algal suspension and mixing time appeared to be 

 about 0.2 sec. 



The entire apparatus was illuminated from each side by a 

 Bine- tube bank of 40-watt fluorescent lights (white) giving 

 a nniform intensity of abwit 2000 footcandles from each side. 

 During an experiment the algal suspension was illuminated 

 for an hour or more with 4% COj beifort the start of the flow 

 C* exposures. Exposures to C"Oj ranging from 1.0 to 16 

 sec. were then carried otit and the products of C'^ reduc- 



2HCHO 

 1.7 



4- 



5HCOOH 

 2, 3, 4, 5. 6 



Sedoheptulose 



+ 



Mannoheptulose 



CO, 

 6 



v + 



6HCOOH 



tion analyzed in the usual way" by paper chromatography 

 and radioautography. 



Short Soybean Experiments. — A single excised trifoUate 

 leaf from a soybean plant (var. Hawkeye) was placed in a 

 circular flat illumination chamber with a detachable lace. 

 The chamber was equipped with two tubes, the lower one 

 leading through a stopcock to an aspirator and the "Pper 

 one tlmMigh a two-way stopcock to a loop oonUining COj. 

 A loosely tied thread led from the leaf stem under the de- 

 tachable face gasket, thence through a boiling ethanol bath 

 and a glass tube to a weight. The illumination chamber was 

 partially evacuated, both stopcocks were closed, and clamps 

 removed from the chamber, the detachable face remaining 

 in position through atmospheric pressure. With the open- 

 ing of the upper stopcock, the C"Oi was swept into the cham- 



(10) A. A. Benson, « •<., This Jootmal, W. 1710 (I960). 



94 



