THE ENERGETICS OF PHOTOSYNTHESIS 



71 



experiments in alkaline medium have been carried out with the one-vessel 

 method. 



a. pH 5, not compensated 



10 min dark 

 10 min ;' 

 10 min dark 

 10 min i' 

 10 min dark 

 10 min i' 



30 min dark 

 30 min /' 



Ao, = +45.6 yul 



Small Vessel 

 Pressure Changes 



— 12.0 mm 



— 5.0 mm 

 — 13.0 mm 



— 4.5 mm 



— 12.5 mm 



— 4.0 mm 



— 37. 5 mm 



— 13.5 mm 



h' = +24.0 mm 

 -VCO2 = —53.0 ixl 7 



-1.18 



Large Vessel 

 Pressure Changes 



— 4.0 mm 



— 1.5 mm 



— 5.0 mm 



— 1.5 mm 



— 5.0 mm 



— 1.5 mm 



— 14.0 mm 



— 4.5 mm 



/; = + 9 . 5 mm 



\/<P = 3.6 



/; . pH 5, compensated 



15 min / 



15 min / + i' 



15 min ;' 



15 min i + i' 



30 min / 



30 min i + i' 



xoi = +41.3 n\ 



h' 



A'COo 



Small Vessel 

 Pressure Changes 



+ 15.5 mm 

 + 29.5 mm 

 + 17.5 mm 

 + 29.0 mm 



+ 33 .0 mm 

 + 58. 5 mm 



= +25 . 5 mm 

 -43.0^1 y 



= -1.04 



Large Vessel 

 Pressure Changes 



+ 11.0 mm 

 + 17.0 mm 

 + 10.5 mm 

 + 16.0 mm 



+ 21.5 mm 

 + 33. mm 



h = +11.5 mm 

 1/<P = 3.9 



c. pH 9.2 {carbonate buffer), not compensated 



A'oj = 0.657 mm^ 



/' = 5.4 /ul quanta/min 



10 min dark 

 10 min i' 

 10 min dark 

 10 min i' 

 10 min dark 



20 min dark (calculated) 

 20 min i' 



Pressure Changes 



— 33 . 5 mm 



— 23. 5 mm 



— 30. 5 mm 



— 22 . 5 mm 

 -30.0 mm 



— 62.7 mm 



— 46 . mm 



+ 16.7 mm 



xo. = AA'o, = 16.7 X 0.657 = 11.0 m1 

 \fv> = i'l/xo. = 5.4 X 20/11.0 = 9.8 



