TETRAPLOIDS 



113 



at the graft level (Fig. 67). Adventitious buds grew up 

 from the callus tissue of the exposed surface. One of 

 these plants was a chimaera, i.e., a plant part of whose 

 tissue was nightshade and part tomato. It was removed 

 and propagated. Some of the axial buds of the new plant 



J 



Fig. 67. 



To the left a, fc, c, methods of grafting of tomato and nightshade. 

 To the right, a periclinal chimaera, S. lycopersicum. (After 

 Winkler.) 



had a tomato epidermis and a nightshade core. These 

 branches were then isolated and planted. The plantlets 

 differed from other chimaeras known to be diploid, which 

 created a suspicion that the new type might have a tetra- 

 ploid core, which was confirmed by examination. The tops 

 of these chimaeras were cut off, and the axial buds of the 

 basal half removed. From the adventitious buds of the 



