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Fig. 2.8 — Stages of restitution in exploded metaphases in the regenerating liver of rats 

 injected with colchicine. Feulgen-fast green staining. A. Eight hours after colchicine. 

 Typical exploded metaphase, without spindle. Scattered and shortened chromosomes. B. 

 Sixteen hours. Chromosome agglutination and lengthening. C. Sixteen hours. Some sug- 

 gestion of catachromatic changes. D. Thirty hours. Formation of large micronuclei; 

 these originate by the catachromatic changes of agglutinated groups of chromosomes. 

 (Original photomicrographs. Courtesy of A. M. Brues, Univ. of Chicago) 



