78 



Colchicine 



under an influence of colchicine are traceable. Finally a quantitative 

 relation between concentration and rate of spindle reduction has been 

 established (Figs. 3.4 and 3.5) . 



3.4: Changes in Spindle Form 



1 he Allniin root tij> cells treated by the research group at Brussels 

 showed that a differentially stainable body was lornied in the col- 



30 



20 - 



10 



LENGTH 



WIDTH 



10 



15 (min.) 20 



H) 



5 - 

 4 - 

 3 - 

 2 - 

 1 - 



O 



RETARDATION 



5^,0-4M ^- 



10 



15 (min.) 2C 



Fig. 3.5 — The shortening of spindle as it disappears differs according to the con- 

 centration. The strong solutions cause rapid disappearance and not much shortening 

 of spindle. The width does not change as much as length of spindle. Measurements 

 of retardation in millicrons show rapid retardation at first, then gradual slowing toward 

 the end. Top group shows decrease in length compared to width for two concentrations. 

 Bottom group indicates the sharp drop at the beginning and slower rates of retardation 

 until final disappearance. (After Inoue) 



chicinized cells.-'' The chromosomes were clustered about this body 

 (Fig. 3.6) . .Such structures persist through the interphase and be- 

 come prominent in the large amoeboid restitution nuclei (Fig. 3.6) . 



Although the relation to spindle was not suggested until later,^^' ^^ 

 the role of the deformed spindle has been mentioned lor a number of 



