

v; 



tS^i* f! 



B 



Fig. 4.2 — Pollen tubes of Po!ygonatum pubescens from cultures in sucrose agar, treated 

 with colchicine and untreated. A. Control culture, poHen tube with generative cell in 

 metaphase, stained with iron acetocarmine. B. Co.chicine mitosis of a diploid species, 

 n-10, to be compared with Figure 2.4D of Chapter 2, the tetraploid species, n-20. C, D, 



E. Reversion to interphase; c-pairs are not separated completely at centromeric region. 



F. Pollen tube c-tumor that is a response to colchicine independen* of any polyploidy. 

 Tube wall staining shows depositions not commonly observed in .. ol. Stained with 

 iron alum haemotoxylin. (Eigsti, 1940) 



