U6 



liquid, the more sorbed pigment formed a zone below lutein and well 

 above cryptoxanthin. The less sorbed pigment fonned a zone just below 

 cryptoxanthin. In columns of magnesia and with petroleum ether plus 

 25 per cent acetone as the wash liquid, the Protosiphon pigment that 

 was the more sorbed in sugar oolxomns formed a red-orange zone below 

 lutein. The less sorbed pigments formed a violet-red zone just above 

 lutein. The sequence of the Protosiphon pigments in the magnesia 

 column was, therefore, the inverse of the sequence in the sugar columns 

 TflB-th respect to spectral absorption and sorbability, these pigments 

 are xanthophylls that contain carbonyl (C«0) groups-'-' ■'•^^■'■'^■'•o 



Cells of Ankistrodesmus falcatus (Chlorococcales) (Appendix IV) 

 cultured voider continuous illumination for several -weeks often varied 

 in color from yellow-green to green. Extracts of the cells from the 

 old cultures yielded a pink-orange zone between the lutein and the 

 chlorophyll a, a similar zone between the chlorophyll a^ and a^' , and 

 another similar zone below chlorophyll a' „ The spectral curves of 

 the pigment from the zone between the lutein and the chlorophyll a^ 

 are reproduced in Figure II, 9o The other, less sorbed pigments ex- 

 hibited similar spectral properties. In columns of sugar and with 

 petroleum ether plus 0,5 psr cent n-propanol, the upper Ankistrodesmus 

 pigment formed a zone contiguous -with the zone of rhodoxanthin. The 

 other Anki strodesmus pigments formed zones much lower than rhodoxanthin 

 in the columno With respect to the spectral absorption cur-ves (Figures 

 11,8 and 11,9) ^^^ "tiie relative sorbability, the supplementary pigments 

 from Protosiphon and from Ankistrodesmus are very much alike 



