- 51 - 



the chlorophyll b zona appeared below the precipitated portion of this 

 xanthophyll zonoo Only after prolonged washing of the column was all 

 the principal xanthophyll carried below the chlorophyll b^ zone, analler 

 quantities of a more sorbed xanthophyll formed a zone above the chloro- 

 phyll bo These two xanthophyll pigments separated in the same sequence 

 when the Euglena extract was adsorbed in a column of magnesia and washed 

 with petroleum ether containing 25 p«r cent acetone. Under these con- 

 ditions, a third pigment appeared below the principal xanthophyll and 

 above the nonsorbed ^-carotene. This third oarotenoid pigment was not 

 sorbed in the sugar oolumn and remained with the ^-carotene.. It was 

 separated rapidly from the (S-carotene by sorption of the carotene frac- 

 tion in a magnesia column. The adsorption spectra of this pigment and 

 of the two unique xanthophylls are shown in Figure 111,2, 



The more adsorbed xanthophyll formed a zone far above lycopene and 

 zeaxanthin in columns of magnesia irtien washed with petroleum ether plus 

 25 p«r cent acetone^^>5. The less adsorbed xanthophyll mixed with lyco- 

 pene and sorbed in a column of magnesia yielded a single zone, but in 

 colijmns of sugar, the xanthophyll was readily separable from the nonsorbed 

 lycopene. In ether solutions, the Euglena carotenoid pi^ents did not 

 yield blue colors ithen treated with concentrated hydrochloric acid. They 

 were not altered by saponification with potassium hydroxide in methanol. 

 The oarotene-lilce pigment was not extracted from petroleum ether with 90 

 per cent methanol, and therefore^ it may be a carotene rather than a 

 xanthophyll. 



Pi gments of the "Golden" Algae (Chrysophyta) of the Class Xanthophyceae 

 or Heterokontae 



The dozen species of the Heterokontae available for these studies 

 were green or yellow=-green organisms (Appendix VI) that yielded the 



