- 55 - 



pigments shown in Figure 111,3" Each species contained chlorophyll 

 a^ several xanthophylls -which, from their relative sorbability, spec- 

 tral properties and color reactions, appear to be hitherto undesoribed 

 carotenoids, and S-caroteneo The xanthophylls have not been encountered 

 in the autotrophic species of any other taxonomic class. 



In the sugar column, a strongly sorbed Heterokontae xanthophyll 

 remained near the top of the sorbent (Figure III,3)o Two xanthophylls 

 that separated slowly from each other and that represented most of the 

 total xanthophyll fraction moved slowly through the columno These two 

 xanthophylls were often preceded by traces of another xanthophyll that 

 formed a light yellow zone. 



The two principal xanthophylls that formed adjacent zones in the 

 sugar column were not separated from each other if the initial zone of 

 the plant extract contained much pigment or if the zones were not washed 

 far along in the column. These two pigments were readily separated, 

 but in the inverse sequence, when the mixture was eluted and readsorbed 

 in a column of magnesia or when the plant extract itself was sorbed in 

 a column of magnesia. In the magnesia column, however, the xanthophyll 

 that had formed the zone second from the top in the sugar column was 

 weakly sorbed and was easily washed through the sorbent o The xantho- 

 phyll forming the zone third from the top in the sugar column was mod- 

 erately sorbed and remained in the upper regions of the magnesia column. 



The spectral absorption curves of the "strongly adsorbed" xantho- 

 phyll (most sorbed in the sugar column), of the xanthophyll "moderately 

 adsorbed" in the magnesia col\imn (third from the top in the sugar col- 

 ijmn) , and of the xanthophyll "weakly sorbed" in the magnesia column 

 (second from the top in the sugar column) are reproduced in Figures Ill^ii. 



