- 55 - 



end III,5» Relative to other xanthophylls, the most adsorbed xantho- 

 phyll from the sugar column was very strongly sorbed being much more 

 sorbed than neoxanthin in columns of sugar and of magnesia, A solu- 

 tion of this strongly sorbed xanthophyll in diethyl ether yielded a 

 blue-green color in the acid layer when shaken with concentrated hydo- 

 chloric acido This blue color faded to yellow in a few minuteSo 



The moderately sorbed xanthophyll from the magnesia column (third 

 in the sugar column) was slightly more sorbed than neoxanthin and zea- 

 xanthin in magnesia with petroleum ether plus 25 per cent acetone as 

 the wash liquid. This xanthophyll was much more sorbed than zeaxanthin 

 in columns of powdered sugar. In sugar columns, it was slightly more 

 sorbed than chlorophyll b and less sorbed than violaxanthin. In solu- 

 tion in ether, it reacted with concentrated hydrochloric acid yielding 

 a light blue-green that faded to yellow in a few minutes « 



The weakly sorbed xanthophyll from the magnesia column (second in 

 the sugar column) was sorbed to the same degree as violaxanthin in 

 columns of magnesia with petroleum ether plus 25 per cent acetone as 

 the wash liquid. But this xanthophyll was less sorbed than violaxan- 

 thin in columns of sugar. Both the weakly sorbed and the moderately 

 sorbed Heterokontae xanthophylls from the magnesia columns (second and 

 third zones. Figure 111,3) formed adjacent zones, in the inverse se- 

 quence, between violaxanthin and chlorophyll b in sugar columns,, The 

 weakly sorbed xanthophyll from the magnesia columns did not yield 

 more than a trace of blue when the ether solution was treated with 

 concentrated hydrochloric acid. 



The minor xanthophylls adsorbed just above and below the two 

 major xanthophylls (Figure 111,3) were strongly sorbed in columns of 



