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CONTINUOUS ELECTROCHROMATOGRAPHY 



Resolution of a Stream of the Mixture 



THR-th flow of the baokgroxmd solution transverse to DC electrical 

 potential, a narrow stream of a solution of the mixture may be added 

 continuously to the migration systemo Under these conditions, the 

 components of the mixture follow separate paths and emerge at separate 

 points at the base of the migration system where they may be collected 

 individually and continuously^* 5* 6, 10^ 



Various modifications of the continuous elect rochromatographic 

 apparatus have been devised. These include tanks packed with powdered 

 solidsj, square paper sheets suspended in a closed atmosphere to prevent 

 evaporation, soft paper pads compressed between glass plates, and soft 

 paper sheets supported upon tilted squares of solidified polystyrene 

 foam^'5, >21,22^ Tapered paper sheets have also been found to provide 

 uniform filtration and effective separation of various ions^^o 



Continuous electrochromatography is effective with various organic 

 ions ranging from proteins to amino acids„ It is useful with all kinds 

 of inorganic ions and with a great variety of substances as the back- 

 ground electrolytic solution^ It produces separations at the lowest 

 concentrations that can be detected^^, a typical continuous separation 

 of silver, cadmium, bismuth, and aluminum ions is provided by Figure 

 V,10, which shows the paths followed by the ions in the migration 

 medium, a tapered sheet of papero 



The separations produced by continuous electrochromatography de- 

 pend primarily upon the selective electrical migration„ They also de- 

 pend upon the selective sorption of the components of the mixture^"^>^o 



