Cyonfemis 



Preface 



V 



I. General Working Conditions, Precautions and 



Prerequisites i 



Glassware ^ 



Utilization of sea-water 4 



Thermometers 5 



Optical equipment 5 



Water immersion lenses 5 



Other accessories ^ 



Literature " 



II. Normal Development 7 



Method for diatom-culture lo 



Indicia for normal development n 



In echinid eggs I^ 



In nereid eggs ^3 



In Asterias eggs I4 



In other eggs ^4 



Polyspermy ^5 



Tempo of development ■. ■ • ■ ^^ 



Tempo of development as criterion for normality i6 



Temperature • ^" 



Evaporation i" 



Seasonal changes ^7 



Literature ^° 



III. Methods for Handling Eggs and Sperm in the 



Laboratory 2° 



General considerations 20 



Notes on special forms 2i 



Arbacia ^^ 



Echinarachnius ^4 



vu 



