CONTENTS ix 



Methods for inducing experimental parthenogenesis 47 



Hypertonic sea-water 4^ 



Increase in temperature 50 



Acid in sea-water 5^ 



Double treatment 5^ 



Literature 5^ 



V. Methods of Fixation 54 



The value of fixed eggs 54 



The choice of the fixative 55 



Fixatives most commonly used on eggs 57 



Mercury bichloride 57 



Picric acid 57 



Chromic acid 5° 



Osmic acid 5° 



Flemming's solution and modifications 60 



Author's method of using Meves' solution. ... 61 



Altmann fixation 63 



Washing 03 



Dehydration 04 



Final stages of dehydration 65 



Literature 05 



VL Methods of Clearing and Imbedding 66 



Clearing 66 



Imbedding 67 



Preparation of the paraffin block 70 



Cutting serial sections 7^ 



Mounting the sections 74 



Preparation of slides for staining 75 



VII. Methods of Staining 7^ 



Intra vitam staining 76 



Staining of fixed eggs 7^ 



In toto staining 7° 



Staining of the sectioned egg 77 



Nuclear stains 77 



Heidenhain's iron haematoxylin 78 



Author's modification of Heidenhain's iron 



haematoxylin • • 7^ 



