necessary to proceed with their preparation as soon as possible 

 after they have been taken from the sea. Certain Companularidae 

 in particular, such as Aglaophenia, Plumularia, Sertularia, and 

 the like, which live in deep water, almost always arrive at the 

 laboratory in a damaged condition or dead. They are more easily 

 injured than other forms by the dredge, the bottom net, or other 

 fishing apparatus. The best plan to follow with such specimens 

 is to put them directly into alcohol, to preserve the perisarc 

 at least. To treat the animals perfectly, they must be attended 

 to on shipboard as soon as caught. 



Other forms which live at less depth, and which can be 

 fished by using every precaution against injuring them, must 

 be handled with gread rapidity, otherwise, after a short time, 

 the polyps become retracted, and it is very difficult to kill 

 them in an expanded state. In general, these forms are more 

 contractile than the Tubularidae. 



All the Hydroidea — that is to say, the permanently poly- 

 poid forms, with very rare exceptions — are killed with hot 

 saturated sublimate. If the polyps are not in complete expansion 

 when received, the colonies should be allowed to expand in beakers 

 of fresh sea water. As soon as the fixing fluid has been poured 

 over the animals the whole should be turned into a crystalizing 

 dish, containing fresh water, to cool; then the animals should 

 be removed to another dish of fresh water for washing, and after 

 five minutes to weak alcohol (50 per cent) . If it is desired to 

 avoid the heating of the liquid, cold sublimate can be used, but 

 only for the Tubularidae. 



Large colonies of Tubularia and Pennaria are killed with 

 the mixture of sublimate and chromic acid in volume equal to that 

 of the water in which they are, and after a few minutes they are 

 washed and removed to alcohol. Antennularia may be killed in 

 cold sublimate, washed in fresh water, and placed in 50 per cent 

 alcohol, and so on. 



Medusa Forms Of The Tubularidae 



The small forms, Eleutheria (Clavatella) , Cladonema, 

 Podocoryne, and the like, are killed with the mixture of sublimate 

 and acetic acid, used in large proportion. Eleutheria may be 

 killed with Kleinenberg ' s solution. 



Lizzia koellikeri and Oceania pileata, as soon as the 

 tentacles have become well distended, are killed with concentrated 

 acetic acid and immediately poured into a tube containing the 

 mixture of alcohol and chromic acid. By gently agitating the 

 tube the animal regains its normal form. After remaining in the 

 mixture about fifteen minutes it is placed in 35 per cent alcohol, 

 and then gradually transferred to that of 70 per cent. Another 



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