lizing dish a stratum of a mixture of glycerin, 1 part, 70 per 

 cent alcohol, 2 parts, and sea water, 2 parts. This stratum will 

 slowly diffuse throughout the water, and after some hours the 

 animals will be narcotized and will remain fully distended, if 

 transferred to alcohol. 



Experience at the station has shown that alcohol alone 

 suffices for the treatment of these worms. Instead of the mix- 

 ture just described, one may add to the sea water 5 per cent of 

 absolute alcohol and let the animals remain therein until they 

 have lost motion, an operation which varies from two to twelve 

 hours for the different species. It is a good plan not to allow 

 these worms to become entirely dead in the sea water. The harden- 

 ing is done in the long wax-bottomed trays with 70 per cent alco- 

 hol, straightening out the animals and holding them in place when 

 necessary by means of wooden pins. After a few hours in the tray, 

 they should be put into tubes and allowed to rest in a horizontal 

 position for a day or so. Since 70 per cent alcohol does not pene- 

 trate the tissues of these animals well enough to prevent macera- 

 tion, 90 per cent alcohol must be used for permanent preservation. 

 Large specimens should be suspended in the tube by means of a 

 thread attached to a float. 



The method just described has given good results with 

 annelids belonging to the following families: Polygordiidae, 

 Opheliadae, Capitellidae, Telethusidae, Maldanidae, Ariciidae, 

 Cirratulidae, Spionidae, Terebellidae, with the exception of the 

 genera Polymnia and Lanice, which are killed with the mixture of 

 sublimate and chromic acid; among the Aphroditidae certain Poly- 

 noinae, and all the Sigalioninae; the Amphinomidae, which can also 

 be well treated with saturated sublimate; among the Eunicidae, the 

 Staurocephalinae, Lysaretinae, and Lumbriconereinae; all the Nereida* 

 Glyceridae, Syllidae, Hesionidae, and Phyllodocidae. 



In the family of the Chlorhaemidae the genera Stylarioides 

 and Trophonia are narcotized with alcoholized sea water, hardened 

 in chromic acid of 1 per cent, and transferred to alcohol. Sipho- 

 nostomum diplochaitos of the same family is killed in a solution 

 of chloral hydrate of 5 per cent, and after hardening for fifteen 

 minutes in 1 per cent chromic acid is transferred to alcohol. 

 Another good method is to use the sulphate of copper and sublimate 

 mixture for killing, allowing the animals to remain five minutes 

 in the solution. Animals of this species, when treated with the 

 ordinary reagents, break to pieces with the greatest ease. 



Hermionidae are immersed directly in 70 per cent alcohol 

 (old solution will do) , taking care that the animals do not die 

 in a curved position. 



Chaetopteridae, Sternaspidae, the large Spirographis and 

 1 the large Serpulinas of the genus Protula are killed in 1 per 



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