FOREWORD 



An important activity of the Systematic s -Ecology 

 Program is the preparation of a study collection of the 

 marine organisms of the Cape Cod Region. Preparation of 

 animal specimens requires careful narcotization, killing, 

 fixation, and preservation. Two very useful manuals in 

 print are: 



Lo Bianco, Salvatore. 1899. The methods em- 

 ployed at the Naples Zoological Station for 

 the preservation of marine animals. Trans- 

 lated from the original Italian by E. 0. 

 Hovey. U. S. Nat. Mus. Bull. No. 39, Part M: 

 3-42. 



Wagstaffe, R., and J. H. Fidler. 1955. The 

 preservation of natural history specimens. 

 Vol. I. Invertebrates. London: H. F. & 

 G. Witherby Ltd. 205 pp., 139 text figs. 



Reprints of the Lo Bianco paper are no longer 

 generally available and the paper lacks an index. Lo Bianco 

 discusses his material by species whereas Wagstaffe and 

 Fidler consider only major taxa. To simplify the work of 

 narcotization and preservation of animals, the following 

 sections have been compiled to serve as a preliminary 

 working guide. Improved methods will be incorporated in 

 these notes as they appear and are solicited. 



Two additional references, not abstracted here, 

 are also useful: {1) British Museum (Natural History), 

 1954. Instructions for collectors: Invertebrate animals 

 other than insects. Great Britain: Adlard & Son, Ltd., 

 76 pp. (2) Guyer, M. F., 1953. Appendix D. Preparation of 

 microscopic material, pp. 283-306, in Animal Micrology. 

 Univ. Chicago Press. 



