in equal parts , and then transferred to 70 per cent alcohol. 



Amphipoda. — All the Laemodipodes , Crevettines and Iperines 

 were formerly prepared by putting them at once into alcohol of 

 70 per cent, but now they are put into Perenyl's solution for 

 fifteen minutes before they are immersed in alcohol. The trans- 

 parent forms of the last division (Phronima, etc.) are killed in 

 sublimate. 



Ispoda. — These are put at once into 70 per cent alcohol, 

 with the exception of the Bopyrides and Entoniscides, which are 

 first placed in a mixture of 90 per cent alcohol and saturated 

 sublimate in equal parts (like the Rhizocephala) or in a saturated 

 sublimate alone. 



Cumacea and Stomatopoda go directly into alcohol, though 

 the transparent larval forms of the latter are first put into 

 saturated sublimate for a few minutes. 



Schizopoda go at once into alcohol or they may be treated 

 first with saturated sublimate. 



To avoid the breaking of the appendages of the Decapoda, 

 these forms are allowed to die in fresh water before they are put 

 into alcohol. They remain in fresh water only as long as is nec- 

 essary, otherwise the membranous appendages are injured. 



2138 — No. 39, Pt. M. 3 



With the Paguridae the alcohol must be changed often, and 

 they are to be preserved permanently in 90 per cent alcohol, be- 

 cause the shell is permeable to only a small degree. 



The larvae of the Decapods (Zoea, Phyllosoma, etc.) are 

 killed in sublimate or with a few drops of osmic acid of 1 per 

 cent. 



Pantopoda 



The Pantopoda are killed in chromic acid of one-half per 

 cent so that they will remain with the legs distended. As they 

 are almost always covered with foreign bodies, it is necessary 

 to let them live for several days in beakers of fresh sea water, 

 so that they may clear themselves of these extraneous growths. 



Mollusca 



Lamellibranchs may be prepared with the valves open by 

 narcotizing them in alcoholized sea water, where they may remain 

 from six to twelve hours or even more, according to the species. 

 The siphonate forms should not be transferred to alchol before 

 they are thoroughly stupefied, otherwise the siphons will contract. 

 After the animals have been narcotized, it is an excellent plan 

 to place them in chromic acid of 1 per cent for about a half hour. 



- 41 - 



