Ether - seawater 



Ethyl acetate fumes 



Ethyl bromide 



Euparal 



Fleming's fluid (1% chromic acid 25 cc 1% 



osmic acid 10 cc glacial acetic 5 cc, 



distilled water, 60 cc) 

 Formalin 4, 6, 10% 

 Formol alcohol 

 Gilson's fluid 

 Glycerin 



Glycerin 1 part, 70% ale. 2 parts, seawater 2 parts 

 *Kleinenberg ' s solution (picric acid, saturated 100 cc, 



sulphuric acid, concentrated 2 cc, filter and add 



3 times the volume of distilled water) 

 Magnesium chloride 

 Magnesium sulphate crystals 

 Menthol crystals 

 Muller's solution (Potassium bichromate 2 g, sodium 



sulphate 1 g, distilled water 100 cc) 

 Perenyi's solution (40 cc of 10% nitric acid, 30 cc 



of 0.5% chromic acid, 30 cc of 90% ale.) 

 Potassium bichromate - osmic acid 

 Prussian blue 

 Schaudinn's fluid 

 *Seawater 

 Sodium chloride solution 

 Sodium hydroxide 10% 

 Stovaine 

 Tobacco smoke 



(B) SOME NARCOTIZING REAGENTS AND THEIR USES 



Most invertebrates are highly contractile and to be 

 preserved in an extended condition must be narcotized 

 slowly in water, either until dead or until they may be 

 killed or fixed without contracting. The following 

 reagents and methods are recommended. 



ALCOHOL. This is advised for the larger invertebrates. A 

 50 or 70% solution is added drop by drop, to the surface 

 of the water. Lo Bianco' s mixture containing 40 parts of 

 70% alcohol, 20 parts of glycerin, and 40 parts of water is 

 also excellent and is poured onto the surface of the water 

 and allowed to diffuse slowly. 



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