B-EUCAINE HYDROCHLORIDE. This is used as a 1% solution 



gradually introduced to the water containing the animals. 

 It is especially valuable for Flosculariae, Vorticellidae, 

 Rotatoria and many larval forms and dissolves in seawater 

 to approximately 0.5%. In the British Collector's Hand- 

 book, the following is recommended; 



Eucaine 1 g 



Alcohol 90% 10 ml 



Distilled water 10 ml 

 to be introduced slowly over a period of time. It is 

 primarily for larger forms. 



CARBONIC ACID GAS. This has been used for Coelenterata, 

 Echinodermata, and Hirudinea. The gas is introduced by 

 squirting the contents of a soda-water bottle into a 

 considerable volume of water containing the animals so 

 that the water is saturated by the gas. 



CHLORAL HYDRATE. Very good rusults have been obtained with 

 this chemical with Actinae, Annelida, Mollusca, freshwater 

 Polyzoa and many larval forms. The crystals should be 

 dropped directly into the water containing the animals, 

 or the animals placed directly in a 5-10% solution. For 

 more delicate work, the solution should be introduced very 

 gradually over a period of several hours. It is used for 

 killing animals in rock crevices, incrustations of calcareous 

 algae, and colonies of serpulas and madrepores. The animals 

 should remain in the solution for 4-6 hours. 

 ADVANTAGE: If animals do not narcotize as desired, they 

 may be replaced in clean seawater, and will recover. 



CHLOROFORM. This is useful for highly contractile animals 

 and should be employed by squirting small quantities of it 

 through a fine syringe every five or ten minutes onto the 

 surface of the water containing the animals. It may also 

 be placed in a separate container and enclosed under a 

 belljar together with the animals to be treated. For some 

 forms one or two drops should be added every 5 or 10 minutes 

 to the vessell containing them. 



CHLORETONE (Acetone Chloroform) . This is recommended as a 



1% solution for small Polyzoa, used in the manner described 

 for chloroform and added slowly. 



COCAINE. 2 g of powder in 100 cc of 5% alcohol is a very 

 good narcotic. 



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