receptacle until only enough remains to cover them. This opera- 

 tion should be performed with great care to avoid any shock 

 which could cause the retraction of the tentacles. Then pour 

 rapidly into the jar a volume of chrom-acetic No. 2 double that 

 of the water in which the animals remain, and immediately after- 

 wards transfer them to alcohol of from 35 to 50 per cent, giving 

 the preparation a few gentle shakes to free the tentacles and 

 dispose them in natural manner. Another good method of killing 

 is with hot saturated sublimate, using the same proportions as 

 of the chrom-acetic mixture, and washing the animals when scarcely 

 dead in fresh water before the transfer to weak alcohol. 



The large Alcyonium is treated in the following manner: 

 After the rapid bath in chrom-acetic No. 2 it is suspended, scarce- 

 ly dead, in a jar containing weak alcohol in such a way that its 

 tentacles do not touch the walls of the jar, and if the polyps 

 remain well distended, the change to the different grades of 

 alcohol then goes forward very gradually. In the weak alcohol 

 minute bubbles of air frequently form and attach themselves to 

 the tentacles, giving them a tendency to float and thus causing 

 distortion. Striking the sides of the receptacle with light 

 blows will rid the tentacles of the bubbles. 



Pennatula phosphorea and Kophobelemnon, when they have 

 become well distended, are taken by the naked base and very 

 swiftly immersed in a tall, cylindrical jar containing the 

 chrom-acetic mixture No. 2, and after a few seconds are placed 

 in a crystallizing dish containing 50 per cent alcohol, where 

 they are allowed to rest on their backs. Then with a small 

 syringe with a very fine point inject alcohol into them through 

 a minute hole made in the extremity of the base. In this manner 

 the alcohol penetrates to all parts of the interior and distends 

 the polyps. Tie a thread around the end of the base above the 

 hole which was made so that the escape of the alcohol may be 

 prevented. After some hours the animals should be transferred 

 to 70 per cent alcohol, and in the final receptacle Kophobelemnon 

 should be suspended upside down by means of a glass float with 

 a hook in it. 



Pennatula rubra, Pteroides spinulosus, Veretillum, and 

 Funiculina are killed like the Pennatulids just mentioned, but 

 no injection is made after the transfer to weak alcohol. Soft 

 forms like Veretillum should be suspended in the final receptacle. 

 Small forms of the Pennatulids may be killed without removing 

 them from the vessel in which they have become distended, and 

 they are then treated like the Cornularians. 



Gorgonia, Gorgonella, Primnoa, Muricea, Isis, etc., should 

 be killed with chrom-acetic mixture No. 2 in the same dish in 

 which they have become distended on account of the great sensiti- 

 veness of their polyps. It is always advisable to have as little 



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