to that of the water in which they are. This should be rapidly 

 poured into the jar containing Actinian, after as much of the 

 water therein has been poured off as may be and leave the animal 

 immersed. A solution which is now much used instead of the 

 chrom-picric mixture just mentioned is made of — 



Chromic acid, of 1 per cent 1 part 



Saturated solution picric acid 1 part 



Formalin, of 4 per cent 1 part 



When the animals die, they will fall from the sides of 

 the glass, and they should then be transferred to another jar 

 containing chromic acid of one-half per cent, where they should 

 be suspended upside down by means of a glass float, the hook of 

 which has been passed through the lower rim of the body. The 

 animals should be gently shaken to give the tentacles a natural 

 position. After half an hour they are placed in weak alcohol, 

 and then gradually transferred to that of 70 per cent. It is 

 a good plan to suspend the animals upside down by means of a 

 glass float, the hook of which has been passed through the 

 lower rim of the body. The animals should be gently shaken to 

 give the tentacles a natural position. After half an hour they 

 are placed in weak alcohol, and then gradually transferred to 

 that of 70 per cent. It is a good plan to suspend the animals 

 upside down by means of a float in the final receptacle, though 

 it is hardly worth while to do this for the smaller specimens. 



The following Actinians may be killed with boiling satu- 

 rated sublimate: Eloactis, Sagartia dohrni, Paranthus, Corynactis, 

 and small specimens of Aiptasia. Before they are transferred to 

 alcohol, the animals should be allowed to harden for some minutes 

 in chromic acid of one-half per cent. 



When Heliactis bellis, Bunodes gemmaceus, and B. rigidus 

 are well distended, two-thirds of the sea water in the jar con- 

 taining them should be removed and its place filled by a chloral 

 hydrate solution 0.2 of 1 per cent. After a few minutes this 

 liquid should be poured off until there is barely enough in the 

 jar to cover the animals, which should then be killed with cold 

 saturated sublimate. 



Adamsia rondeleti is narcotized with tobacco smoke in the 

 following manner: Remove the hermit crabs from the shells on 

 which the actinias are growing and kill them in fresh water. 

 Suspend the shells by threads in beakers of sea water in which 

 the actinias will have ample room for expansion. The thread may 

 be wound around the shell or passed through a hole in it and 

 then tied over a stick of wood which rests on the edge of the 



- 24 - 



