beaker. Place one or more of the beakers in a shallow tray 

 (preferably with flat bottom and perpendicular sides) contain- 

 ing a little water and cover with a bell glass. Fill the bell 

 glass by means of the bellows and pipe described on page 9, 

 being careful at the same time to insert a U-shaped piece of 

 glass tubing under the edge of the bell glass to permit the 

 escape of the confined air as the smoke is forced in. Avoid 

 jarring the glasses Containing the actinias. 



To regulate properly the duration of the whole operation 

 it is necessary that the first fumigation should be made about 

 2 o'clock in the afternoon. Little by little the smoke clears 

 up and the water begins to absorb the narcotizing substances 

 contained therein and the animals for the most part distend 

 the corona of tentacles. About 5 o'clock a second fumigation 

 like the first should be made, and the objects are allowed 

 then to remain overnight. The following morning carefully 

 remove the bell jar and touch the tentacles with a needle to 

 learn in what condition of sensibility they are. If they do 

 not contract under this stimulus, place a small open beaker 

 containing a few cubic centimeters of chloroform beside the jar 

 containing the actinia and replace the bell jar, allowing the 

 fumes of the chloroform to work for two or three hours. Lastly 

 the animals are killed in the chrom-acetic mixture No. 2, harden- 

 ed with chromic acid of one-half per cent and placed in weak 

 alcohol and so on, where they are to remain suspended. If the 

 tentacles give signs of sensitiveness, make a third fumigation 

 and after a few hours test again. This is the only method 

 which has proved successful in obtaining specimens with the 

 column well distended and with the disk and tentacles in full 

 expansion. Cold weather retards this and other narcotizing 

 processes in a very marked degree. 



Adamsia palliata can be prepared in the same manner with- 

 out suspension. Good results have also been obtained by narcotiz- 

 ing the animal slowly with alcoholized sea water and then killing 

 with the chrom-acetic mixture No. 2, or with hot saturated sub- 

 limate. 



Cladactis, Cereactis, and the little Bunodeopsis strumosa 

 are killed with the chrom-acetic mixture No. 2, and immediately 

 afterwards hardened in chromic acid of 1 per cent. The first 

 two should be suspended in the hardening and the preserving fluids 

 by means of a glass float, the hook of which has been passed 

 through the margin of the base. Before beginning operations, 

 see that the specimens of Cladactis and Cereactis are perfectly 

 sound and especially that they are not torn or cut, otherwise 

 when they are placed in alcohol the liquid contents of the body 

 will exude through the rents. Large specimens of Cerianthus are 

 fixed with acetic acid and immediately afterwards are suspended 



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