tyroligneus - USE RARE 



falphuric - USE RARE. (Ingredient of Kleinenberg ' s solution) 



Alcohol - Most indispensible liquid. 



1) For preparation and preservation of transparent 

 animals, filter and dilute with distilled water. 



2) Caution - 



a) if reused, acids and alkalies must be neutral- 

 ized. 



b) to avoid bubbles, mix previous to using. 



3) Use 70% for preserving - 90% for special cases. 



4) Soft and gelatinous animals must remain 2-6 hours 

 in 35, 50, 60, 70% alcohol each. 



5) Animals may be placed in alcohols or if too deli- 

 cate siphon used alcohol and flood them with in- 

 creasing strengths. 



6) 70% used for permanent preservation. 



7) Alcohol is effective for narcotizing and "killing 

 animals slowly or quickly. 



Copper Sulphate 



1) Use - killing larvae and delicate forms in a 

 5-10% solution of hot freshwater used alone or 

 mixed with corrosive sublimate. 



2) Caution - wash objects frequently with water or 

 they will not remain clear. Treat with acid, if 

 specimens become opaque. 



Corrosive sublimate 



1) Fixing agent. Permeates and hardens tissues 



quickly. 



2) Use in concentrated solution in either fresh or 

 seawater - hot or cold. 



3) Caution - 



a) do not use metal tools; they decompose and 

 stain specimens, 



b) make with hot water, 



c) do not boil in open vessel - vapors are very 

 poisonous, 



d) keep hands out of solution especially if then- 

 ar e cut. 



Formalin 



1) Used to preserve animals temporarily only, 

 because animals eventually decompose in it. 



- 56 - 



