METHODS FOR NARCOTIZING AND PRESERVING MARINE INVERTEBRATES, 

 ABSTRACTED FROM WAGSTAFFE AND FIDLER, ARRANGED BY MAJOR TAXA 



PROTOZOA, FORAMINIFERA, RADIOLARIA 



Not narcotized, but killed, fixed and stained by various 

 methods . 



PORIFERA 



Not narcotized. 



(a) Wash in clean salt or fresh water. 



(b) Immerse in 95% alcohol if small; if large, use 

 only small piece for preserving. 



(c) Store in 95% alcohol. 



(d) Spicules 



1) Siliceous 



a) Boil in nitric acid to get rid of soft 

 parts. 



b) Pour off acid and wash several times with 

 distilled water, filter, dry in an oven 

 and mount on a slide in euparal. 



2) Calcareous 



a) Macerate in 10% caustic soda. 



b) Wash as above, filter, dry and mount 

 on a slide in euparal. 



(e) Larvae 



1) Killing and fixing. Place in little seawater 

 and flood for 2 or 3 minutes with Flemming's 

 fluid (see page 52) 



CNIDARIA (Coelenterata) 



A) Hydroids 



1) Place in seawater and allow to extend. 



2) Use menthol, magnesium sulphate or chloral hydrate 

 crystals in a 5-10% solution: Add over a period 



of 3 + hours until animals are unresponsive to touch, 



B) Medusae 



1) Place in a shallow dish with a little seawater 

 and allow to expand. 



2) Use menthol, magnesium sulphate or chloral hydrate 

 crystals in a 5-10% solution, stovaine or chloro- 

 form. 



3) Caution - Do not let them die before preserving 



them. 



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