C) Siphonophora 



1) Caliconectae 



a) Kill with saturated picric acid. 



b) Store in 10% formalin. 



2) Physonectae 



a) Narcotize with menthol. 



b) Kill with 10% formalin, or a saturated 

 solution of corrosive sublimate. 



c) Add Flemming's fluid (see page 52) or 

 Kleinenberg ' s fluid (see page 52) and 

 leave specimens in it 24 hours. 



d) Store in 70% alcohol. 



3) Cystonectae 



a) Kill with corrosive sublimate and glacial 

 acetic acid (95 ml corrosive sublimate 

 saturated solution in distilled water and 

 5 ml glacial acetic acid) . When dead, 

 place in 1/2% for 1 1/2 hours, 30% alcohol 

 for 1/2 hour, 50% alcohol for 1 hour. 



b) Store in 70% alcohol. 



4) Disconectae 



a) Kill with saturated picric acid or corrosive 

 sublimate solution. When dead add a little 

 chromic acid and leave for 1/2 hour. If kill- 

 ed with picric acid, place in 30% alcohol for 

 2 hours, 50% alcohol for 1 hour. If killed 

 with corrosive sublimate, wash well with 

 running water and pass through alcohols as 

 above . 



b) Store in 70% alcohol. 



D) Scyphomedusae 



1) Kill with a 5% solution of formalin in seawater. 

 In 10 minutes add enough 5% solution of picric 

 acid to color the formalin. Stir contents over 

 a 24 hour period and increase the formalin to 10%. 



E) Actinozoa 



1) Alcionaria 



a) Narcotize with menthol crystals. 



b) Store in 70% alcohol. 



2) Zoantharia 



a) Allow specimens to expand in seawater. Sprinkle 

 in a few crystals of menthol or chloral hydrate, 

 5-10%, use 3% alcoholized seawater or bubble 

 tobacco smoke through specimen container. 

 Leave over night. 



- 59 - 



