water for 2-3 hours or until movement is barely 

 perceptible. 



2) Kill by passing animals through 35, 50% alcohol. 



3) Store in 70% alcohol. 



B) Trematoda 



1) Clean by shaking specimens in a 1% salt solution. 



2) Kill by adding an equal (equal to the amount of 

 salt solution) amount of hot (about 60 °C) satu- 

 rated aqueous solution of corrosive sublimate. 



3) Fix in this solution for 1-2 days. 



4) Wash in running water for 4-5 hours and pass 

 through 50% alcohol. 



5) Store in 70% alcohol. 



C) Cestoda 



1) Clean by washing in a 1% salt solution. 



2) Kill by immersion in a 1% aqueous solution of 

 chromic acid after wrapping around a glass bottle 

 or dish. When dead, place in formol-acetic alcohol 

 for 24 hours. Wash in 50% alcohol. 



3) Store in 70% alcohol. 



NEMERTEA 



A) Allow to expand in running or standing seawater. 

 Narcotize in a 5-10% solution of menthol or chloral 

 hydrate crystals. 



B) Caution - Watch carefully that specimens do not vomit 

 proboscides. 



C) Killing. 



a) After resting in standing seawater for 10-15 

 minutes, draw off most of water and flood with 

 an aqueous solution of corrosive sublimate and 

 leave specimens in this for 15-20 minutes. 



b) Use almost boiling water and transfer to corrosive 

 sublimate for 10 minutes. Wash in several changes 

 of 50% iodized alcohol. Pass to 70% alcohol. 



D) Store in 70% alcohol. 



ROTIFERA 



A) Slow Narcotization. Place specimens in 8 ml water in 

 a watch-glass. Add 2 drops of a 2% aqueous solution 

 of benzamine hydrochloride. Mix with a ccarse pipette. 

 After 20-30 minutes, add 1 or 2 drops of benzamine 

 hydrochloride (as much as 6 drops may be needed if 

 water is acid). Total time, 1-3 hours. 



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