B) Rapid Narcotization. Place specimens in less water 

 than above. For every ml of water, add 3-10 drops 

 of: 



2% aqueous solution of benzamine hydrochloride .... 3 parts 



water 6 parts 



Pure cellosolve (ethylene glycol mono-ethyl ether).. 1 part 

 Total time, 3 + minutes. 



C) Killing. 



a) with 4 ml of 10% formalin. 



b) with very hot water. Allow specimens to expand 

 in cold water in a watch-glass; then flood with 

 hot water and transfer to 10% formalin. 



NEMATODA 



A) Extraction. Wash out gut with a 1% aqueous salt solution. 



B) Cleaning. Place washings in a glass jar of 1% aqueous 

 salt solution and shake well. When nematodes sink to the 

 bottom, decant saline and replace with new saline. 

 Continue this process until specimens are clean. 



C) Killing. Pour off saline and add 70% alcohol for 1-2 

 minutes . 



D) Store in fresh 70% alcohol. 



ANNELIDA 



A) Polychaeta 



1) Place in clean seawater and add 70% alcohol drop 

 by drop until specimens no longer respond to touch. 

 Tubicolous forms will leave tubes if menthol crystals 

 are sprinkled on their water surface and left over 

 night . 



2) Killing. Arrange parts on a dry glass plate and 

 press behind the head to force the extension of 

 the proboscis. Flood with formol -alcohol for 



20 minutes. Place in a flat dish with formol -alcohol 

 for 24 hours. 



3) Store in fresh formol-alcohol. 



B) Hirudinea 



1) Allow to expand in water and from time to time add 

 crystals of chloral hydrate or magnesium sulphate. 



2) Killing. When specimens are unresponsive to touch, 

 straighten out on a glass plate and flood with warm 

 10% formalin. 



3) Store in 70% formol-alcohol. 



C) Gephyra 



1) Add a few crystals of chloral hydrate or magnesium 

 sulphate from time to time. 



- 62 - 



