2) 



3) 

 4) 



Killing. Place on a glass plate. Extend probuscis 



and flood with 70% formol-alcohol. 



Store in 70% formol-alcohol. 



Larvae. Killing and fixing. Draw off most of the 



water and flood with Flemming's fluid (see page 52) 



for 2-3 minutes. 



CRUSTACEA 



A) Branchiopoda, Ostracoda, Copepoda. 



1) Kill with 5-10% formalin for 10 minutes. Transfer 

 to 50% alcohol for 10 minutes. 



2) Store in 70% alcohol or 5-10% formalin. 



B) Branchiura 



1) Kill by dropping into caustic soda (NaOH) . 

 Transfer to 50% alcohol. 



2) Store in 70% alcohol. 



C) Cirripedia 



1) Allow to expand in water and narcotize with menthol 

 crystals. 



2) Kill in 10% formalin or when no longer responsive 

 to touch use hot (60 °C) saturated solution of 

 corrosive sublimate for 10-20 minutes and wash in 

 70% iodized alcohol. 



3) Store in 70% alcohol. 



D) Malacostraca 



1) Decapoda 



a) Kill by placing specimens in cold freshwater 

 with or without chloroform. Drop small forms 

 into 70% alcohol. 



b) Store in 70% alcohol with a little glycerin 

 added. 



2) Larval Stages 



a) Kill by placing animals in Bouin's fluid for 

 3-6 hours. Wash in several changes of 30% 

 alcohol for 30 minutes; 50% alcohol for 30 

 minutes. 



b) Store in 70% alcohol. 



3) Isopoda 



a) Kill by dropping specimens into 100 ml alcohol 

 i 4 ml glycerin. They may remain in this for 

 10-14 days. 



b) Transfer and store in 70% alcohol. 



MOLLUSCA 



A) Narcotizing substances and groups 

 1) Acetic acid and seawater. 

 a) Tritonia 



- 63 - 



