10) Magnesium sulphate crystals, allowed to dissolve 

 in a bag in with the animals, or free in their 

 container . 



a) Nudibranchiata 



11) Menthol crystals. 



a) Amphineura 



b) Lamellibranchia 



c) Scaphopoda 



12) Seawater, standing. 



a) Aeolidiidae 



b) Atlantidae 



c) Elysiidae 



d) Heteropoda 



e) Hyaleidae 

 ft Idalia 



g) Philine 

 h) Pteropoda 

 i) Polycera 

 j) Tethys 

 k) Triopa 



13) Killing and or preserving. 



a) Alcohol. Place specimens in 50% alcohol 

 for 48 hours and transfer to 70%. 



1) Amphineura 



2) Lamellibranchiata 



3) Scaphopoda 



b) Chloroform, ether or ethyl bromide. 

 Inject into mantle cavity. 



1) Cephalopoda 



a) Preserve by placing specimen on 

 board and pin down arms. Inject 

 5% formalin into mantle cavity and 

 place animal in 10% formalin to 

 harden. Wash in running water. 

 Remove from board. 



b) Store in 70% alcohol or 10% formalin, 



POLYZOA (Bryozoa) 



A) Narcotizing. Place specimens in salt or fresh-water 

 depending on the species. Use menthol, chloral hydrate 

 5-10%, stovaine or B-eucaine hydrochloride (the last, 

 especially for freshwater forms) . When polyps no longer 

 respond to touch, draw off water and replace with 5% 

 formalin. 



B) Store in 5% formalin. 



C) Cleaning. Transfer specimens to distilled water. Remove 

 foreign matter with a soft camel's hair brush and wash 

 several times in clean, distilled water. 



- 65 - 



