D) Larvae 



1) Narcotize as above and fix in hot (60°C) Bouin's 

 fluid. Wash in water. 



2) Store in 70% alcohol. 



BRANCHIOPODA 



A) Narcotize by adding alcohol to specimen's seawater to 

 make a 5% solution. 



B) Kill by leaving specimens in this seawater for 30 

 minutes . 



C) Store in 70% alcohol. 



CHAETOGNATHA 



A) Narcotize by adding menthol or chloral hydrate crystals 

 to small amount of seawater containing specimens. 



B) Killing. Straighten specimens out on a piece of glass 

 and flood with Bouin's or Gilson's fluid. 



C) Storage. If fixed in Gilson's fluid, wash in 2-3 

 changes of 50% iodized alcohol. If fixed in Bouin's 

 fluid wash in 2-3 changes of 50% alcohol and in both 

 cases store in 5% formalin. 



ECHINODERMATA 



A) Asteroidea 



1) Narcotize by adding crystals of magnesium sulphate 

 to their seawater (narcotize with tube feet upper- 

 most) . 



2) Kill by placing specimens in 70% alcohol. 



3) Store in 70% alcohol. 



B) Ophiuroidea 



1) Narcotize specimens in seawater to which weak 

 alcohol is gradually added. 



C) Echinoidea 



1) Kill by placing specimens quickly in 70% alcohol 

 or strong acetic acid. If acetic acid is used, 

 wash out well in running freshwater. 



D) Holothuroidea 



1) Narcotize in fresh water to which crystals of 

 menthol or magnesium sulphate have been added. 



2) Kill after narcotized by immersing in 15% formalin 

 for a few minutes and immediately inject 70% alcohol 

 into body cavity. 



E) Crinoidea 



1) Killing. Place animals head down in a dish of 

 seawater and hold in position by pressing down. 

 Flood with 90% alcohol for 3-4 minutes. 



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