F) Larvae 



1) Narcotizing. Place in seawater and add a few 

 chloral hydrate crystals or stovaine. 



2) Killing. Draw off most of seawater and flood 

 with Bouin's fluid. Leave for 2-3 hours and 

 wash with water. 



TUNICATA 



A) Narcotize in clean seawater to which crystals of menthol 

 or chloral hydrate have been slowly added or 3% alcoholi- 

 zed seawater. 



B) Killing. Draw off most of seawater containing animals 

 and flood with Bouin's fluid or a saturated solution of 

 corrosive sublimate and leave for 1-24 hours. 



C) Storage. If fixed in Bouin's fluid, wash in several 

 changes of 50% alcohol. If fixed in corrosive sublimate, 

 wash in several changes of 50% iodized alcohol. In 

 either case store in 70% alcohol. 



- 67 - 



