aqueous stains readily. Prior to freezing, specimens relaxed 

 in Sevin recover from the treatment with either Sevin and Sevin CO2 

 when returned to running seawater. Deep freezing is required to 

 Kill the animals in a relaxed condition as treatment with Sevin 

 does not insure complete insensibility. This method of narcoti- 

 zation and killing, as well as slow and/or rapid deep freezing 

 alone, may be useful for other highly refractory organisms and 

 should be tested on them. 



Fixation and preservation ; Animals may be fixed in 

 formalin, washed and stored in 1% proplene phenoxetol-5% 

 glycerol, or may be fixed and preserved in 70% alcohol. 



Reference: (1) Carriker, M. R. , J. W. Blake 1959. A 

 method for full relaxation of muricids. Nautilus 73: 16-21. 



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