AMINO ACIDS , PEPTIDES AND PROTEINS 229 



Another development of major significance was the demonstration by Nirenberg and 

 Matthai (82) that DNAse treated cell-free preparations will synthesize polypeptides in 

 response to synthetic polynucleotides. Thus, the addition of polyuridylic acid led to the 

 formation of polyphenylalanine. By using synthetic polynucleotides composed of more 

 than one N-base and making the assumption that a triplet code is involved it has been 

 possible to devise a nucleotide code for the amino acids that are incorporated into proteins. 

 It is to be anticipated that future experiments will permit the elucidation of the nucleotide 

 sequence requirement for every amino acid. 



GENERAL REFERENCE 



Alexander, P. , and Block, R. J. , Lab oratory Manual of Analytica l Methods of Protein 



Chemistry , 3 Vols. , Pergamon Press, N. Y. , 1960-1961. 

 Fowden, L. , "Non-Protein Amino Acids of Plants", Endeavour 21 35 (1962). 

 Greenberg, D. M. , ed. , Metabolic Pathways , Vol. IT, Academic Press, N. Y. , 1961. 

 Greenstein, J. P. and Winitz, M. , Chemistry of the Amino Acids, 3 Vols., John Wiley 



and Sons, Inc., New York, N. Y. , 1961. 

 Meister, A., Biochemistry of the Amino Acids , Academic Press, New York, N. Y. , 



1957. 

 Paech and Tracey 4^ (Several Articles). 

 Kuhland 8 (Several Articles). 



Scheraga, H. A., Protein Structure , Academic Press, New York, N. Y. , 1961. 

 Webster, G. C. , Nitrogen Metabolism in Plants, Harper and Row, N. Y. , 1959. 

 Advances in Protein Chemistry , Vol. I, 1946 to present, Academic Press, New York, 



N. Y. 



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