TABLE OF CONTENTS 



Introduction 



By James G. Needham, Cornell University 



General Methods of Collecting, Maintaining, and Rearing 

 Marine Invertebrates in the Laboratory 

 By Paul S. Galtsoff, U. S. Bureau of Fisheries 



i. Collecting 



2. Marine Aquaria 



3. Artificial Seawater 



4. Methods of Securing Food for Marine Invertebrates 



5. Methods of Obtaining a Culture of a Single Food Species 



Collecting and Rearing Terrestrial and Freshwater Invertebrates 

 By F. E. Lutz, J. G. Needham, and P. S. Welch 



1. Collecting and Handling Living Specimens 



Aquatic Animals 

 Aerial Insects . 



2. Cages and Shelter 



3. Cage Management 



Feeding and Watering . 

 Temperature and Humidity 

 Sanitation 



PAGE 



3 



5 



13 

 27 



3 1 

 36 



40 

 41 

 44 

 46 

 48 

 48 

 48 



49 



Phylum I. Protozoa 



Class Mastigophora 



Growth of Free-Living Protozoa in Pure Cultures . . . 51 



By R. P. Hall, University College, New York University 



Cultivation of Protozoa 59 



By John P. Turner, University of Minnesota 



Polytoma Cultures 61 



By Josephine C. Ferris, University of Nebraska 



Culturing Euglena proximo, 61 



By J. A. Cederstrom, University of Minnesota 



A Culture Medium for Free-Living Flagellates (Reprinted) . .62 

 By James B. Lackey, U. S. Public Health Service 



vii 



