Culture of Single Species 39 



In order to insure an ample supply of food, the cultivation of diatoms 

 or other algae should be started at least two weeks before the time of the 

 experiment to be conducted with marine larvae. Between the seasons of 

 experimental work with marine larvae, a small number of stock cultures 

 of their food should be maintained in the laboratory. 



Bibliography 

 Allen, E. J. 1914. On the culture of the plankton diatom Thalassiosira gravida, 



Cleve, in artificial seawater. J. Mar. Biol. Assoc. 10:417. 

 Allen, E. J., and Nelson, E. W. 1910. On the artificial culture of marine plankton 



organisms. Ibid. N. S. 8:421. 

 Bond, R. M. 1933. A contribution to the study of the natural food-cycle in aquatic 



environments. Bull. Bingham Oceanog. Coll. 43, Art. 4:1. 

 Browne, E. T. 1898. On keeping Medusae alive in aquarium. J. Mar. Biol. Assoc. 



5:186. 



1907. A new method of growing Hydroids in small aquaria by means of a 



continuous current tube. Ibid. N. S. 8:37. 



Burch, A. B., and Eakin, R. M. 1934. A device for water circulation. Science 



80:563. 

 Dittmar, W. 1884. "Challenger" reports. Physics and Chemistry 1. 

 Forchhammer, G. 1865. On the composition of seawater in the different parts of 



the ocean. Philos. Trans. 155. 

 Galtsoff, P. S. 1932. The life in the ocean from a biochemical point of view. J. 



Wash. Acad. Set. 22:246. 



1934- The biochemistry of the invertebrates of the sea. Ecol. Monog. 4:481. 



Galtsoff, P. S., and Cable, L. 1933. The current rotor. Science 77:242. 

 Galtsoff, P. S., Prythercii, H. F., Smith, R. O., and Koehring, V. 1935. The 



effects of crude oil pollution on oysters in Louisiana waters. Bull. U. S. Bur. Fish. 



48:143. 

 Gran, H. H. 1931. On the conditions for the production of plankton in the sea. 



Cons. Perm. Intern. Pour V Exploration de la Mer. Rapp. et Proces-Verbaux des 



Reunions. 75:37. 



1932. Phytoplankton methods and problems. J. du Cons. Intern. Pour 



V Exploration de la Mer. 7:343. 



1933. Studies on the biology and chemistry of the Gulf of Maine. Biol. Bull. 



64:159. 

 Grave, C. 1902. A method of rearing marine larvae. Science 15:579. 

 Hagmeler, A. 1925. Neue Aquarium-einrichtungen der Staatlichen Bioligischen 



Anstalt Auf Helgoland. Intern. Revue d-gesamt Hydrobiol. u. Hydrographic 



12:405. 

 1933. Die Ziichtung verschriedener Wirbelloser Meerestiere in Abderhalden's 



Handbuch d. Biol. Arbeitsmethoden Abt. 9 T. 5, 1:465. 

 Harvey, H. W. 1928. Biological Chemistry and Physics of the seawater. Cambridge 



University Press. 194 pp. 

 Herbst, C. 1903-4. Ueber die zur Entwicklung der Seeigellarven Notwendigen 



anorganischen Stoffe, ihre Rolle und ihre Vertretbarkeit. Arch. f. Mech. 17:306. 

 Hopkins, A. E. 1934. A mechanism for the continuous circulation and aeration of 



water in small aquaria. Science 80:383. 

 Johnstone, J. 1908. Conditions of life in the sea. Cambridge University Press. 



332 PP. 

 Just, E. E. 1928. Methods of experimental embryology with specific reference to 

 marine invertebrates. The Collecting Net. 3:1. 



