54 Phylum Protozoa 



table 2. Media for colorless flagellates. 



Media 



Constituents G H I J 



Glycine 2.0 gm. 



Peptone 2.0 gm 10. o gm 10.0 gm. 



Gelatin iS°-° g m - 



NH4NO3 0.5 gm. 



Dextrose 2.0 gm. 



Sodium acetate 2.0 gm 2.0 gm 2.0 gm. 



K2HPO4 0.2 gm 2.0 gm. 



KH2PO4 0.25 gm 1.5 gm. 



K2CO3 2.0 gm. 



KC1 0.25 gm. 



MgS0 4 0.1 gm 0.25 gm 0.25 gm. 



Distilled water 1.0 1 1.0 1 1.0 1 



Tap water 1.0 1 



table 3. Media for ciliates. 



Media 



Constituents K L M N P 



Peptone 10. o gm . . . 10. o gm. . . 10-30 gm 5.0 gm. 



KH 2 P0 4 2.0 gm. 



K2HPO4 0.02 gm. 



Na 2 HP0 4 0.01 gm. 



NaCl 0.5 gm. . . .4.0 gm 0.003 g m - • °- 02 S m - 



KC1 0.01 gm. 



MgSC"4 0.01 gm 0.025 g m - • 0.0045 g m • °-° 2 § m - 



CaCl. 0.01 gm 0.001 gm. 



KNO3 0.5 gm 0.0013 gm. 



(NH 4 ) 2 P0 4 0.05 gm. 



FeCU 0.001 gm. . .0.0002 gm. 



CaS0 4 0.015 g m - 



Ca(N0 3 ) 2 0.2 gm. 



FeS0 4 Trace 



Distilled water . .1.0 1 1.0 1 1.0 1 1.0 1 1.0 1 1.0 1 



for the same species. Lwoff adjusted the pH to 8.0 with K2CO3. 

 Medium I was used by Jahn (1933) for the growth of stock and experi- 

 mental cultures of Chilomonas Paramecium. Medium J has been used 

 in our laboratory for maintaining stock cultures of Astasia ocellata (Jahn 

 strain), Astasia sp. (Jahn strain), and Chilomonas Paramecium. 



MEDIA FOR CILIATES 



The growth of Colpidium striatum and C. campylum in various types 

 of media has been investigated by Elliott (1935a), and that of Glau- 

 coma ficaria and G. piriformis by Johnson ( 1936). Results obtained by 



