Cryptomonadidae 59 



Oehler, R. 1916. Amobenzucht auf reinem Boden. Arch. f. Protist. 37:175. 



1919. Flagellaten- und Ciliatenzucht auf reinem Boden. Ibid. 40:16. 



1920. Gereinigte Ciliatenzucht. /bid. 41:34. 



1924. Weitere Mitteilungen iiber gereinigte Amoben- und Ciliatenzucht. 



Ibid. 49:112. 



1924a. Gereinigte Zucht von freilebenden Amoben, Flagellaten, und Ciliaten. 



Ibid. 49:287. 

 Parpart, A. K. 1928. The bacteriological sterilization of Paramecium. Biol. Bull. 



55:ii3. 

 Phelps, A. 1934. Studies on the nutrition of Paramecium. Arch. j. Protist. 



82:134. 

 Pringsheim, E. G. 1921. Zur Physiologie saprophytischer Flagellaten (Polytoma, 



Astasia und Chilomonas). Beitr. Allg. Bot. 2:88. 

 1928. Physiologische Untersuchungen an Paramecium bursaria. Arch. j. 



Protist. 64:289. 



1928a. Algenreinkulturen. Eine Liste der Stamme, welche auf Wunsch 



agbegeben werden. Ibid. 63:255. 



1930. Neue Chlamydomonaceen, welche in Reinkultur gewonnen wurden. 



Ibid. 69:95. 

 Sandon, H. 1932. The food of Protozoa. Publ. Fac. Sci. Egypt (Cairo, Misr-Sokar 



Press), 187 pp. 

 Tstjjitani, J. 1898. Ueber die Reinkultur der Amoben. Zentralbl. f. Bakt., I, 



24:666. 

 Wulker, G. 1911. Die Technik der Amobenzuchtung. Ibid. 33:314- 



Order cryptomonadida, 

 Family cryptomonadidae 



CULTIVATION OF PROTOZOA 



John P. Turner, University of Minnesota 

 Chilomonas sp. To each 100 cc. of pond water* (previously freed from 

 Protozoa by heating to 70 C.) add 4 grains of wheat. Inoculate with 

 Chilomonas from old culture or from wild stock after isolating them 

 with a fine pipette [see pp. 43, 7°] • Within a week the culture should be 

 cloudy with Chilomonas, and this serves as a valuable source of food for 

 many larger forms.- Chilomonas may be obtained from almost any 

 pond where there is decomposing vegetation, especially if that be allowed 

 to stand in water in the laboratory for a few days with wheat added. 



Amoeba proteus. To each 100 cc. of pond water (previously heated 

 to 70 C. if a "pure" culture is wanted) add 2 grains of wheat and a few 

 drops of Chilomonas culture to serve as food. A day or two later inocu- 

 late with the Amoeba (often found on dead lily pads, etc., in shallow 

 water). Keep between 15 and 25 C. for best results, and disturb as 

 little as possible. Cultivate in water less than an inch deep. 



* Note: In most parts of the country tap water from city mains will grow Protozoa 

 satisfactorily if allowed to stand for a few days after being drawn. In many cases pond, 

 lake, spring, or stream water is better. 



