Euglenidae 61 



References 



For the culture of Chilomonas see also pp. 62, 63, and 136. 



For the culture of Chilomonas paramedian see pp. 53 and 113. 

 Order Phytomonadida 



For the culture of various members, of this order see p. 52. 

 Family Chlamydomonadidae 



For the culture of Chlamydomonas see p. 53. 



For the culture of Haematococcus pluvialis see p. 53. 



For the culture of Chlorogonium euchlorum and C. elongation see p. 52. 



For the culture of Carteria see pp. 35 and 38. 

 Family Volvocidae 



For the culture of Volvox see references on p. 72. 



Family polytomidae 



POLYTOMA CULTURES* 



Josephine C. Ferris, University of Nebraska 



PUT 2 70 grams of chicken-size bone meal into a muslin bag and tie 

 compactly so that the whole mass is in a firm ball. Cover with water 

 and bring to a boil. Pour off water and allow the bag to cool for 2 hours. 

 Boil 1 gram of ground timothy hay for 10 minutes and allow to cool. 

 Pour cooled hay solution into small battery jar and add enough cold 

 sterilized water to make 1200 cc. Lift cooled bag of bone meal with 

 sterilized forceps into jar and inoculate with Polytoma. Keep at about 

 1 7 to 20 C. Every 48 hours repeat the above procedure and use all the 

 scum of the old culture in inoculating the new culture. Such a culture 

 may be maintained many months or even years. Removing the surface 

 film of Polytoma with a sterilized spoon and centrifuging yields con- 

 centrated masses of these Protozoa. 



References 



For the culture of Polytoma see also p. 53. 



For the culture of Parapolytoma satura see pp. 113 and 116. 



Order euglenoidida, Family euglenidae 



CULTURING EUGLENA PROXIMA 



J. A. Cederstrom, University of Minnesota 



Euglena proximo, may be cultured very readily for laboratory use in 

 filtered rain water (100 cc.) to which is added 1 cc. of one day old 

 pasturized milk from which the cream has been removed completely. 

 Keep the culture at ordinary room temperatures of from 6o° to 68° or 



* See Biol. Bull. 63:442. 1932- 



