64 Phylum Protozoa 



finger bowl and the Vorticellae are introduced; great numbers of the 

 animals will be found clinging to the glass surface within two weeks. It is 

 advisable to subculture every three weeks. 



Stentor. This organism may be cultured by two methods; namely, 

 that found useful for Amoeba, and that described for Vorticella, except 

 that certain modifications must be made here. The rice-agar with 50 cc. 

 of Solution A [see footnote on p. 73] is permitted to stand for two days in 

 a finger bowl ; at this point Chilomonas is added together with as many 

 Stentors as possible. Or, the medium of y 2 gram of mashed hard-boiled 

 egg yolk in 750 cc. of distilled water is permitted to stand for three days, 

 filtered, and inoculated with Chilomonas and the Stentors. (About 5 cc. 

 of a heavy Chilomonas culture are necessary for the inoculation in both 

 cases.) It is desirable to subculture every month. 



Stylonychia and Oxytricha. For these hypotrichs and certain others 

 the presence of Chilomonas seems advantageous. Before introducing the 

 desired ciliate, allow some 30 cc. of Solution A in a rice-agar bowl, 

 inoculated with 10 cc. of a Chilomonas culture [see p. 63] to stand 

 about four days. Swarming cultures have usually been obtained within 

 two weeks. 



References 



For a Phacus-like organism see p. 69. 



For the culture of Colacium vesiculosum see p. 53. 



For Euglenamorpha see p. 69. 

 Family Astasiidae 



For the culture of Astasia sp. and A. ocellata see p. 54. 



For the culture of Peranema see pp. 62 and 136. 

 Family Heteronemidae 



For the culture of Entosiphon see pp. 62 and 177. 



Order protomonadida, 

 Family trypanosomatidae 



A SIMPLE METHOD FOR CULTURING TRYPANOSOMA 



LEWISI 



Reed O. Christenson, University of Minnesota 



IT is not always feasible, on short notice, to obtain living trypanosomes. 

 Laboratory cultures should be established and maintained. This can 

 be done easily by using the following method which we have found both 

 simple and reliable. 



Wild rats are caught by setting a number of spring-traps near their 

 holes or runways. The captives are killed and opened. A small amount 

 of blood is taken from the heart, using clean pipettes, and this is dropped 



