Polymastigida 67 



SPECIES CULTURED 



Retortamonas intestinalis (Waskia or Embadomonas) grew well on 

 both Locke-egg and ovomucoid, forming cysts in each medium. On the 

 Locke-egg it lived from 3-10 days. One culture lived 17 days on the 

 ovomucoid. The cultures were grown at 35 C. (Hogue, 1921a). 



A new variety of Retortamonas found in man grows well on sodium 

 chloride sheep serum water. It has been kept at room temperature (20- 

 33 C.) for over 3 years and has survived one heat wave of 39. 5 C. 

 It is transferred every 7 days. "Deep cultures" of it are kept at room 

 temperature. In them the organisms live from 8-9 months. One culture 

 lived over 12 months (Hogue, 1933). 



Trichomonas buccalis grew on a sodium chloride sheep serum medium 

 especially rich in sheep serum and containing a small amount of human 

 saliva. On this medium they were cultured for over 7 months. Transfers 

 were made every 2-3 days, though they lived about 5 days on this 

 medium at 30 C. In "deep cultures" they lived from 30-60 days 

 (Hogue, 1926). 



Trichomonas hominis grew well on Locke-egg, ovomucoid and sodium 

 chloride sheep serum water. In the early work the first two media were 

 used and the cultures were incubated at 35 C. They lived about 6 days 

 on the Locke-egg and from 6-10 days on the ovomucoid (Hogue, 1921b). 

 In later work sodium chloride sheep serum water has been used. They 

 have been incubated at 36 C. and at room temperature. In "deep 

 cultures" these organisms live from 35-66 days at 35 C. The most 

 favorable pH for rapid multiplication is pH 7.2-8.4 with pH 8 as an 

 optimum. If it is desirable to keep the cultures longer pH 7.2-7.4 is 

 better. Here they divide more slowly (Hogue, 1922a). 



Spirochacta eurygyrata grew well on Locke-egg, ovomucoid, and 

 sodium chloride pig serum water. On ovomucoid and Locke-egg they 

 lived from 8-12 days. The best results were obtained with sodium 

 chloride pig serum water with pH 7. These were incubated at 35 ° C. 

 In "deep cultures" of sodium chloride pig serum w T ater they lived from 

 60-90 days. One culture contained active spirochaetes 127 days after 

 inoculation (Hogue, 1922b). 



References 

 For the culture of Trichomonas see also pp. 69, 88, and 91. 

 For the culture of Tritrichomonas see p. 118. 

 For the culture of Enteromonas see p. 88. 

 For the culture of Monocercomonas see p. 129. 

 For the culture of Chilomastix see p. 88. 



Bibliography 

 Hogue, M. J. 1921a. Waskia intestinalis : Its cultivation and cyst formation. 

 J. A. M. A. 77:112. 



