72 Phylum Protozoa 



takes place by adding fresh manure solution, they serve as admirable 

 sources of material for inoculating new cultures. 



Paramecium. Hay cultures are perhaps the most common type but 

 Paramecium will thrive in almost any medium which does not become 

 too sour and which will grow plenty of bacteria. Cracked wheat has 

 been used; also rice, rolled wheat or oats, bread, and malted milk. The 

 latter makes a good bacterial culture; such cultures do not last long 

 but they are easily inoculated into new cultures. If wheat is used, add 

 boiled wheat to distilled water at the rate of 30 or 40 kernels to a gallon 

 of water. Rolled wheat or oats should be used raw, and at the begin- 

 ning not more than 10 flakes to a pint of water. Bread should be used 

 sparingly. 



Bibliography 



Dawson, J. A. 1928. The culture of large free-living Amoebae. Amer. Nat. 62:453. 

 Hyman, L. 1925. Methods of securing and cultivating Protozoa. 



I. General statements and methods. Trans. Amer. Micr. Soc. 44:216. 

 — 1931. Methods of securing and cultivating Protozoa. 



II. Paramecium and other ciliates. Ibid. 50:50. 



Jennings, H. S. 1903. Methods of cultivating Amoeba and other Protozoa for 



class use. /. A ppl. Micr. and Lab. Methods 6:2406. 

 Kofoid, Charles A. 1915. A reliable method for obtaining Amoeba for class 



use. Trans. Amer. Micr. Soc. 34:271. 

 La Rue, G. R. 1916. Notes on the collection and rearing of Volvox. Ibid. 35:151. 

 1917- Notes on the culturing of microscopic organisms for the zoological 



laboratory. Ibid. 36:163. 



191 7. Further notes on the rearing of Volvox. Ibid. 36:271. 



Smith, B. G. 1907. Volvox for laboratory use. Amer. Nat. 41:31. 



Turtox Service Leaflet No. 4. The care of Protozoan cultures in the laboratory. 

 General Biological Supply Co. 



Turtox Protozoa Booklet. General Biological Supply Co. 



Turtox Biology Catalog and Teachers' Manual. General Biological Sup- 

 ply Co. 



Welch, M. W., 1917. The Growth of Amoeba on a solid medium for class use. 

 Trans. Amer. Micr. Soc. 36:21. 



CULTURE OF SOME FRESHWATER RHIZOPODA 



Paul Brandwein, Washington Square College, New York University 



Amoeba* The following method has been notable in giving a larger 

 proportion of successful cultures which achieve a very dense maximum 

 growth in 3-4 weeks and do not require subculturing for 8-10 weeks. It., 



♦Chalkley. H. W., 1930, Science 71:441 and Pace, D. M., 1933, Arch. f. Protist. 

 79:133 have previously reported two methods for Amoeba. Our medium differs little 

 from Chalkley's, but the method in its entirety has given better results. 



