88 Phylum Protozoa 



Craig, 1930. 



i 2 3 



Inactivated Inactivated Inactivated human 



human blood human blood blood serum i part 



serum i part serum i part 0.85% NaCl 7 parts 



Locke's solution 7 parts Ringer's solution 7 parts 

 Rice starch is added. 



All are sterilized by Berkefeld filtration. 



Tanabe's medium is satisfactory for a certain length of time, but as 

 yet does not justify its use in preference to the Boeck-Drbohlav medium 

 or one of its modifications. However, we feel that their effort to simplify 

 the medium is a step in the right direction. 



Tanabe and Chiba, 1928. 



SLANTS LIQUID 



Agar 10 grams 5% rabbit serum in Ringer's. 



Asparagin 1 gram Rice starch is added. 



Ringer's solution 1000 cc. 



We have not had as much success with Cleveland's liver-infusion-agar 

 medium as was hoped. We agree with him that it seems to be almost 

 specific for Endamoeba histolytica, but we find two objections to its use: 

 1 ) Blastocystis multiplies very rapidly in it, and, 2 ) production of gas 

 by bacteria displaces the agar slants to an annoying degree. The first 

 objection seems to us of considerable importance where only fresh smear 

 examinations are made. 



Cleveland and Collier, 1930. 



SLANTS LIQUID 



Liver Infusion Agar Horse serum 1 part 



(Digestive Ferments Co.) 30 grams 0.8% NaCl 6 parts 



Na2HP04 3 grams 



Water 1000 cc. 



Rice flour is added. 



We have not, as yet, had opportunity to duplicate all details of 

 Deschiens' medium, but it is probable that the results would be some- 

 what similar to those of Tanabe. 



Deschiens, 1930. 



SLANTS LIQUID 



Agar 20 grams Locke — Ringer's solution or 



NaCl 5 grams Physiological salt solution 



Beef extract 2-5 grams 



Water 1000 cc. 



Powdered fish muscle or powdered beef muscle is added to each tube. 



Trichomonas, Chilomastix, Enteromonas, and Embadomonas grow 

 well in the L. E. A., L. E. B., or L. E. S. media. Giardia has not as yet 



