Bursariidae 129 



rather that division is not frequent enough to permit weekly subcultur- 

 ing without gradually diminishing the number of organisms to the point 

 of extinction. Longer intervals between subcultures result in an over- 

 growth of bacteria and the small flagellate Monocercomonas orthopter- 



orum. 



References 



For the culture of Blepharisma see p. 60. 



For the culture of Spirostomum ambiguum and 5. teres see p. 60. 



For the culture of Nyctotherus cordijormis see p. 69. 



Bibliography 

 Lucas, C. L. T. 1928. A study of excystation in Nyctotherus ovalis with notes 



on other intestinal Protozoa of the cockroach. J. Paras. 14:272. 

 Smith, N. M., and Barret, H. P. 1928. The cultivation of a parasitic Anioeba 

 from the cockroach. Ibid. 14:161. 



M. E. D. 



Family Bursariidae 



A METHOD FOR CULTURING BURSARIA TRUNCATELLA* 



Amos B. K. Penn, Tsing Hua University, Peiping, China 



ADD 1 gram of timothy hay, 1 gram of rye, and 5 grams of fresh cab- 

 l\ bage to 600 cc. of spring water. Boil slowly for 5 minutes. Let 

 stand uncovered for 2 days to allow development of bacteria, then re- 

 move the cabbage, add 500 cc. spring water, transfer 250 cc. of the solu- 

 tion with corresponding amounts of hay and rye to each of several % 

 liter jars and inoculate with Paramecium, Colpidium, and Chilomonas. 

 After 2 or 3 days, i.e., when these organisms have become abundant, 

 inoculate with Bursaria. Cover the jars and keep them in indirect sun- 

 light at room temperature. 



If a film of gummy substance has developed on the surface of the in- 

 fusion, break it. If no gummy substance is present, add some from an 

 old culture. 



A culture thus prepared reaches a flourishing condition (iohh individ- 

 uals per cc.) in 2 or 3 days, and continues in this condition for 3 or 

 4 days. If the infusion is more dilute, the cultures flourish longer, but 

 the Bursaria does not become so abundant. 



References 

 For the culture of Bursaria truncatella see also p. 103. 

 For the culture of Balantidium coli see p. 118. 

 Family Stentoridae 



For the culture of Stentor see pp. 64 and 134. 

 For the culture of Stentor coeruleus see pp. 60 and 103. 

 Order Oligotrichida 



* Reprinted from Anat. Rec. 54:99, 1932, at suggestion of the author. 



